Cornelian cherry is also looking at its antioxidant capacity.When I apply ABTS method, the results are negative in both 1/100 trolox and samples.Is there a protocol related to this method?
Dear Şeydanur Bayoğlu,
Please refer to the following paper for ABTS method that we tested in Apricot, Apple and strawberry fruits.
https://link.springer.com/article/10.1007/s13197-019-03850-2
The above cited Article could be reached at the following link:
https://rdcu.be/bGzLi
Duried Alwazeer how much ABTS solution and potassium persulfate do you combine together before incubation?
Dear Brittany,
For preparing 10 ml of ABTS (7 mM) - potassium persulfate (2.45 mM) solution:
2 ml (12.24 mM) potassium persulfate was added to
38.4 mg ABTS, then the volume was completed to 10 ml with sodium acetate (20 mM).
This ABTS solution was then stored in the dark at room temperature for 12–16 h.
This solution must be used within two days.
Good lucks
Duried
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