Can I embed samples in 30% sucrose in agarose and paraffin?

08 September 2023 0 4K Report

Hi,

the dehydration machine was faulty and destroyed some of my samples embedded in agarose that were meant to be dehydrated and embedded in paraffin.

i have a whole set of backup samples, which unfortunately are already infiltrated in 30% sucrose in 1X PBS. Could I embed them in agarose -> paraffin anyway or would it be safer to just proceed with the cryo protocol?

thanks a lot for your advice!

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