Reg- Biochemical assay ?

We did biochemical assays like esterase and phosphatase (quantitatively) activity in the whole body homogenate of the larval extract. For that, first we have to estimate the protein concentration...

10 March 2019 9,348 2 View

please any one give valuable suggestion what are problems need to be address in content based classification and retrieval of image?

i am doing research in Content based image classification and retrieval using visual bag of feature. my doubt is how to do denoise the image in CBIR

03 June 2018 9,659 5 View

Now i am going to do antibacterial activity using crude plant extract so for that how do i make diffrent concentartion..

Now i am going to do antibacterial activity using crude plant extract so for that how do i make different concentration of 10,20,30ug/ml from stock 1mg/mL. but to my knowledge i am using following...

26 July 2017 165 4 View

How to remove excess organic ligands from QDs?

I am having problems in making completely dried QDs films. It always remains sticky. I think it is because of the excess organic ligands (Palmitic acid, TOP, Oleic acid). I purified the QDs by...

21 March 2017 1,787 4 View

How can I determine the right orientation of windings in a distributed winding stator?

I have a stator(PMSM) with distributed windings in it. I need to determine whether the windings are wound in the correct direction. Is there a method to do it? The stator has 144 slots with 2...

22 June 2014 8,492 2 View

What software can I use to draw unit cell structure?

I would like to draw unit cell structures such as wurtzite and cubic. Is there any free softwares to draw unit cells. Attached image is the model which I want to draw.

19 June 2014 9,677 37 View

Can anyone help with indexing a SAED ring pattern?

I have recorded the SAED pattern of my sample. It has a ring pattern and the scale bar is 5 1/nm. Please give me information about how to index SAED pattern using reciprocal scale bar.

02 February 2014 2,711 4 View

Dynamic Surface Electromyography (sEMG) - Processing techniques, analyzing methods and its important characteristics

I am trying to learn about the muscle characteristics of a particular neurodegenerative disease: 1) What kind of information (i.e muscle weakness, Spasticity, fatigue) should I look out for in...

14 January 2013 2,434 9 View

Is it possible to use EMG data, collected using Vicon motion capture system, in Matlab for signal processing?

I am working on Gait analysis. I need to do EMG signal processing and it looks like Matlab is a good option for that. But I have collected data through Vicon motion capture systems and is it...

08 November 2012 4,937 12 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Agarose gel purification pre MinION sequencing?

Dear all, we performed an agarose gel purification with MinElute kit (QIAGEN) to extract bands between 3500 bp and 6000 bp for further sequencing with MinION. After the purification process,...

16 February 2021 5,178 1 View

What is the difference between Q sepharose column and DEAE Sepharose column?

I am working on protein (lipase enzyme) purification. After using the Sephadex G-25 fine column, which column (Q or DEAE sepharose column) should I use? What are the buffers should I select? Thank you

16 February 2021 6,921 5 View

Why samples do not run in resolving gel?

Hello everybody, I have a problem with SDS-PAGE and I do not know why my samples stop at the top of the resolving gel and they do not run through it. The percentage of gel is 15% and the size of...

14 February 2021 9,116 6 View

Low DNA yields with midiprep and maxiprep plasmid extraction kits but excellent results with miniprep kits. What can be a possible reason?

I need to extract large amounts of plasmid DNA for transfection into mammalian cell lines with as little as possible enterotoxins and I usually use the Qiagen Endofree plasmid maxi kit for the...

09 February 2021 3,779 6 View