I got this plasmid from Addgene (https://www.addgene.org/83481/) that I want to put into some mouse immortalized myeloid cells.
The basticidin resistance gene is driven by human phosphoglycerate kinase 1 promoter (hPGK). Will this be a problem?
Most likely there would not be a problem.
Hi,
I agree with @Mukesh that the human PGK promoter will not be a problem.
Mukesh Kumar Gupta Walid Hassene Hamri Thank you Mukesh and Walid for conforming that!
Siamak Gholamalipour
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