Can EDTA 0,5M be treated with DEPC?

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How can upload an article research gate ?

I want to upload my articles drafts

29 June 2024 2,016 3 View

How transmittance is dependent on the band gap and atomic structure?

UV-Visible NIR analysis

20 June 2024 9,056 3 View

How can a single photon propagate in a straight-line in a dielectric medium?

Given the well-documented quantum Rayleigh scattering of single photons in a dielectric medium [1] A. P. Vinogradov, V. Y. Shishkov, I. V. Doronin, E. S. Andrianov, A. A. Pukhov, and A. A....

17 June 2024 7,692 9 View

Which is the best model for hypoxia in cell culture ( MSC or fibroblasts)?

I am tring to culture cells in hypoxia using Cobalt chloride model , but I want to compare with antoher method. Our research is about circRNA expression - qPCR and nanopore oxford sequencing.

05 June 2024 2,246 1 View

Why do you name scientists just A. Silva, as there are multiple scientists with the name Silva whose name starts with an A. ?

You sended me a couple of guestions "Did A. Silva author this work". I Looked at the article and found that just the University of Lisbon has over 10 scientist with that name.

13 May 2024 3,524 0 View

ARMAX model to Transfer Function?

Hi i have estimated an armax model using python sippy library. The estimation gives me two transfer functions H and G. How can I combine them into a single one to predict model output for new...

20 January 2024 1,221 2 View

How the negative photoconductivity of crystal effect the generating radiations from crystal especially terahertz radiation?

Crystal field

07 January 2024 2,849 2 View

Parasite Eggs Identification, can anyone identify this eggs?

Hello, I'm working on my MSc project related to Parasitology of Marine mammals, particularly cetaceans. I've been identifying some eggs and adult specimens by morphological methodologies, however,...

23 December 2023 2,042 1 View

Foundation models as knowledge-based systems?

Hello, during the recent emergence of LLMs and foundation models I restarted thinking about the very early machine learning attempts to incorporate human expertise and knowledge into expert...

22 November 2023 5,625 0 View

Why won’t PEG stay neutralized?

Upon receiving PEG we titrate to find its base value so it can be neutralized with HCl for certain processes. It’s typically basic on the micromolar level (I think from residual KOH or another...

10 November 2023 6,960 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

How to draw the five chelate rings of [Co(EDTA)]- on this picture?

Is this circle one of the rings?

03 August 2024 5,988 0 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Addition of EDTA during the synthesis of copper nanoparticles to prevent it from being oxidized?

I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...

28 July 2024 8,027 3 View

How is surfactant addition calculated?

I want to make 100 ml of MWCNT/distilled water mixture at a volume fraction of % 0.1. MWCNT density is 2100 kg/m3. I will add gum arabic as surfactant. If I add surfactant at a 1:1 ratio, will the...

19 July 2024 8,494 0 View

Size-biased recovery of total RNA isolated with Trizol vs RNeasy?

So - my lab did a bunch of RNA isolations from tissue culture cells, for purposes of RNA-seq, and submitted them to a core facility. We got back a notification that the RINs were too low, and we...

14 July 2024 641 1 View

How can EDTA be removed from already extracted DNA samples?

Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...

13 July 2024 1,675 3 View

How i can calculate mathematically COD concentration if i add 1gr from glucose to 1 L distilled water ?

how i can calculate mathematically COD concentration if i add 1gr from glucose to 1 L distilled water

08 July 2024 9,104 2 View

Is DNase I (RNase-Free) necessary to be used before cDNA synthesis?

I want to isolate RNA from bacillus subtilis using the QuickRNA Fungal Bacterial Miniprep Kit from Zymo Research and further reverse transcriptase to cDNA using the ReverTra Ace qPCR RT Master Mix...

27 June 2024 2,951 5 View