Annealing means selenization (Se Powder/ Se pellets) on RbIn spray deposition surface.
Try 0.7 percentage of the melting point of the base substrate.
Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...
22 July 2024 9,761 5 View
I have prepared Copper thin films by magnetron sputtering and the samples have been annealed at 100, 200 and 300 degree celsius. The variation of the rms roughness and all other AFM height related...
11 June 2024 9,774 1 View
I am doing my undergrad thesis that requires me to do PCR-RFLP analysis on DNA that I extracted from some blood samples. Initially, I was using the Qiagen mastermix for the optimization of my...
14 May 2024 4,370 4 View
Hi, I'm currently trying to generate a one-vector plasmid system for a knockout using CRISPR/Cas9. I'm using lentiCRISPRv2 (ampicillin-resistant) and following the GeCKO Target Guide Sequence...
29 April 2024 3,660 0 View
I recently designed the qrtpcr primers from the 3'utr of the plant's genomic DNA keeping in mind that the annealing temperature should be 60 degree. Once I received the primers first to optimise...
25 April 2024 5,935 3 View
Ligation Independent Cloning literally says no need Ligation, but it still needs ligase to anneal the fragment to vector. And the Protocol used T4 DNA Ligase for anneal process. What does this...
18 April 2024 1,494 3 View
Hi, I have been trying to decide on the best annealing temperature for the F/R primers using a sample. From left is the ladder, 61c, 63c, 65c, no template and the ladder. The expected product size...
11 April 2024 7,592 9 View
I did PCR of 6 amplicons and when I did agarose gel electrophoresis, they all appear consistently bigger than they should be (e.g. the amplicon should be 4000 bp, but is around band of 5000 bp...
09 April 2024 4,487 5 View
When I simulate the NMOS model in ATHENA, when I want to anneal in nitrogen atmosphere after n+ ion implantation, the annealing operation is not possible when the annealing temperature is lower...
08 April 2024 2,798 0 View
Hi All, I am trying to amplify mitochondrial 16S gene for marine snails (Calliostomatidae) and other vetigastropods, but I only get primer dimers or nothing on the gel. These primers have worked...
03 April 2024 8,736 4 View