Can anyone tell me some protocols to determine the period of the estrous cycle in rats using vaginal smears?

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Hi, does anyone have this book?

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Nei Moreira

You can also use another coloration techniques, as Panotic, Schorr or May-Grünwald Giemsa. All of them have good results.

Mijail Rojas-Carvajal

Thank you Nei. Do you have some protocol for the use of these techniques?

Jean-Nicolas Audet

This article has good info on rat estrous phase cycling: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842002000400008&lng=en&nrm=iso&tlng=en

You can use vaginal cotton swabs, as used in dogs or cats, with the same protocol.

Thank you Nei. I've already made it. But we had to develop our own strategy.

Thereza C. M. De Lima

Check the files attached.

In mice.

Another useful paper.

The last one...