i am a research student and need a proper mechanism of action of DEN when introduced into mice, for my research work
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The way Hot pressing can be employ for thermoplastic multilayer, any such process or techniques for thermosets as well?
29 May 2024 504 3 View
which regression analysis will be applied if there are 7 nominal dependent variables(YES/NO categories) and 1 ordinal independent variable(3 categories: Low, normal, high)?
23 April 2024 9,971 3 View
While making forest plot Revman I label the plot as Left control and right experiment. I see that the data supports the control as the diamond is on the left. But when I change labels the...
25 February 2024 3,144 2 View
How grain size effects the antibacterial properties?
30 January 2024 6,225 1 View
Techniques Tools required Step by step Procedure
21 January 2024 8,960 0 View
In CSt how do we calculate Power handling capacity of h plane horn antenna
12 December 2023 1,713 0 View
I've tried the liquid exfoliation method but I'm not getting the results. If anyone else has tried through this method can anyone share protocols so that I can check where I'm making mistakes in...
10 September 2023 3,753 0 View
Hi everyone, I have problem while saving the computed points after adding the break lines in 2d unsteady flow. here i have attached the image of the problem I'm facing.
07 September 2023 2,235 0 View
I am conducting CV analysis of a rGO-based electrode using CHI 650E workstation with platinum foil as counter electrode, an Ag/AgCl reference electrode, and 1M H2So4 electrolyte. How can I decide...
07 September 2023 5,403 4 View
I got negative potential window in the range of -0.6 to 0.3 for 3 electrode system ,so can I use negative voltage window for -0.6 to 0.4 for 2 electrode supercapacitor system.
03 August 2023 1,765 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View