Ok, I carried out theoretical calculation of MO constants and then sensitivity values of multilayers that I optimized through modeling and simulation. Note that no single sensitivity definition was available in literature when comparing the performance of SPR and MOSPR sensors side by side. Motivated by this problem, for the first time in biosensing history, I (in collaboration) systematically benchmarked and highlighted differences between the SPR responses in noble metal, and transition metal, and noble/transition metal bilayers. This serves the general readership who is not familiar in the topic to contextualize the results. I then benchmarked the common sensitivity metric that permits direct comparison of SPR and MOSPR responses at the optimal operating point. Have not seen this result yet? Feel free to download through the following webpage (free of cost):
http://www.mdpi.com/2304-6732/5/3/15
This sensitivity metrics should form the foundation for comparing two different types of sensors based on propagating surface plasmons which was so far not possible to do, to the best of this authors knowledge.
3) Drew schematics to build a lab for magneto-optics (started from nothing)
4) Ordered components through vendors
5) When components arrived, I built the lab, carried out optical alignment, and did what an optical engineer would do in a lab to build a functional laboratory (It was fun to take the challenge, although it was not easy to start in the beginning but the job is successfully done)
6) Successfully tested the functionality of the sensor in air and water media. It was great fun to demonstrate the physics of magneto-optics using the home-built lab.
7) Now, I would like to move on to testing bio-samples (enough test is already done with air and water media). Can anyone suggest where should I start for bio-sensing test with real samples? The sensor is based on propagating surface plasmon resonance. Thanks.
Congratulations on your paper in Photonics. In order to test it for bio samples, initially you have to explore your analyte. Once it is chosen, you have to find out the best functional material which would lead to your best testing on bio-samples. In fact, I am also interested in your endeavor and accordingly expect your outcomes of this research.
Thanks Rajib Biswas. I have additional questions.
So now I have purchased streptavidin, biotin, and avidin from the vendors, and I would like to conjugate one of them into the sensor surface and detect another. I have been through several literature, but I am still undecided where to start from (Clearly, I see the importance of collaboration between Engineers, Chemist, Biology and Physics-- 21st century's need). Can anyone suggest the procedure for this bio-conjugation and any additional information about this whole process? Thanks in advance. If you have any questions about my research, feel free to contact me. I’ll be happy to discuss.
Appreciate if any one you have answers for my questions: Alexandre G. Brolo, Drew A Hall, K.L. Kavanagh, R. Karki, N. Rajasekaran, Richard Weihrich, Rajendra Gyawali, Parashu Ram Kharel, Ali Eftekhari
, Frank Ko,Rosa Alejandra Lukaszew
, Kai Liu , Pritish Mukherjee , and those who have expertise on it.-Conrad ,
Thats really great news, congratulations.
You should find out your targeted analytes (biomarkers to see any disease in the media) and start finding their respective internationalizing groups (ligands/bio-receptors).
Learning never ends! I encourage everyone to be courageous in responding to the questions asked by the seekers. The more knowledge we would distribute to the community, the more it would grow. Its the only thing (knowledge) in this material world that grows bigger as we spend it more, where rest of the material world works totally different way. Thanks.
- Conrad
Thanks Afaque Manzoor I would need a bit more specific answer based on the 3 protein samples I have recieved now .
Bio-conjugation and Sensing:
You might be curious where I stand now since I posted my questions some 19 days ago. I understand researchers are very busy people and I have no complaints that I did not receive many responses to my question. However, when I posted the same question on LinkedIn, one of the researchers working in bio-conjugation responded positively.
Bio-sensing and bio-imaging are hot topics. I am starting my test with a very simplistic protein sample, and then I would move onto more complex protein samples.
Thanks, everyone and has a great day.
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