27 July 2023 1 2K Report

As one of 16s rRNA sequences giving only 70% query cover. What could be the major issues with the sequencing? Suggest some laboratories in India for bacterial genome sequencing and it's price.

More Mittu Koshy's questions See All
Time dilation equation lucid and peremptorily verified?

The time dilation equation, fundamentally in an absolute and correct manner, reduces to square root function in the positive domain and range of real numbers. The anomaly with this function, it is...

29 April 2023 8,120 4 View

How to size a PEM electrolyser , if the demand is 200Nm3/h ?

Hello I am currently trying to figure out how to size a 200Nm3/h capacity PEM electrolyser. How do I design the electrolyser mathematically to get the current density, power rating , cell...

20 July 2022 3,031 0 View

What are the popular techniques for pre-processing measurement data in Smart Grid/Power System?

The data collected from various measurement devices often contains erroneous values - such as noise, missing values, etc. What are the popular techniques for pre-processing measurement data in...

14 February 2021 5,079 2 View

How to predict missing data?

What are the various techniques to predict missing data in power systems? Mostly referring to the measurement data coming in from sensors or smart meters. References are welcome. Thanks in advance!

19 January 2021 6,998 4 View

How to identify missing /bad data in PMU data?

PMU data can go missing due to several factors - faulty PMU or PDC or communication channel, cyber attack, etc. Bad data can be also caused due to some of the above reasons or maybe due to some...

28 December 2020 8,545 3 View

Can anyone share machine (generator) data for IEEE 30 bus system?

I require the machine (generator) data for H, Rs, Xd, Xtd, Xq, Xtq, Td0, Tq0 and D; as well as the exciter data (kA, TA, kE, TE, kF and TF). If you can share the machine data file or link, it will...

22 December 2020 2,721 3 View

Does PMU based state estimation require the system parameters?

When using PMU data for estimating the state of power system (basically, a generator), are the system parameters required?

14 December 2020 5,445 3 View

What is a scoping review?

In what context should we choose to do a scoping review? How is it different from systematic review, literature review, mapping review and Cochrane review? What are its mandatory or optional...

05 July 2020 8,532 3 View

If I use REVEL score as a meta-predictor for clinical variant classification, what would be a reasonable cut-off to filter disease variants?

Ioannidis et al., 2019, reported the development of the REVEL score (rare exome variant ensemble learner) for redefining pathogenic variant classification, and Tian et al., 2019, reported that...

29 March 2020 2,696 2 View

What are the significant updates included in C37.118.1a2014 standard for PMUs?

PMUs are manufactured worldwide by many manufacturers and they are employed in various locations, basically for the monitoring of the system. What are the significant standards to be followed in...

24 February 2020 8,517 1 View

Similar questions and discussions
Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How effective is the Citi Bloc standard basket in enhancing the accuracy and comparability of international construction cost assessments?

Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...

04 August 2024 8,997 1 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

29 July 2024 4,570 0 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View

Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...

28 July 2024 6,938 1 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close