How to dissolve Pentachloronitrobenzene?

Pentachloronitrobenzene is used to prepare the "Nash & Snyder" media to isolate the Fusarium. But this is not dissolving in 75% ethanol and distilled water (Normal and hot both). So Please...

05 February 2021 687 1 View

Why marketers need a forensic understanding of consumers and a creative, agile mindset?

02 February 2021 6,579 10 View

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Connecting With the New Digital Customer Immersive technologies

02 February 2021 614 9 View

How can we know the exact soil taxonomy of any particular site. Is there any online available database for Indian soil taxonomy based on geography?

Please If one can let know the soil taxonomy for vindhyan region falling in Mirzapur district of Uttar Pradesh, India.

14 December 2020 9,879 14 View

What could be the possible reason for lack of Mtb protein expression cloned in pET28a even after induction ?

12 December 2020 2,584 8 View

How can calculate dielectric and electrical properties of nano ferrite powder form ?

How can calculate dielectric and electrical properties of nano ferrite powder form ? from powder form can we calculate electrical properties like dielectric resistivity .

03 December 2020 3,671 4 View

UV Vis of ferrites?

how can calculate band gap using Uv-Vis . ferrites not solvable in water . In which solution disperse the ferrites for Uv vis.

10 November 2020 3,283 3 View

For measure Uv vis of nano ferrites like zn and mg which solvent is used?

For measure Uv vis of nano ferrites like zn and mg which solvent is used

25 October 2020 2,931 4 View

Pre-post intervention study with control group - what types of statistical analysis methods would suit?

Hello all, My research study is an Intervention before and after study ( non-randomised) with a control group. I have one intervention group n=30 with multiple clinical variables and patient...

13 July 2020 1,026 13 View

If we cross Diploid plant (Female Parent) x Tetraploid plant. How much chances of getting viable seed?

In case of guava crossing of Diploid (Psidium guajava, 2n = 22) with P. Molle (2n=44). Is there any chance to get viable seed set?

16 June 2020 9,317 3 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

Is it possible to use maize mature seed to do transformation for maize transgenic study?

Almost all research reports which i ever seen work on Early mature embryo for Agrobacterium mediated maize transformation. In other grass families like rice, we can use mature seed and what do...

17 February 2021 1,434 13 View

Dealing with BMDM seeding and RNA extraction ?

biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...

15 February 2021 1,102 5 View

Can any one explain why the ethyl ester Biodiesel (FAEE) has higher than that of the metyl ester FAME- both of them are derived from the same oil?

Biodiesels derived from waste vegetable oil transesterification with Ethanol (FAEE) show higher peroxide values than those derived by transesterification with methanol (FAME) prepared under the...

13 February 2021 193 6 View

Is secondary metabolite production more at vegetative growth stage or during reproductive stage?

Hi, I am working on the effect of heavy metals on a medicinal plant by pot experimentation. Analyzing the effect of heavy metals on secondary metabolites is one of the parameters. I will be...

11 February 2021 6,683 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Seeds harvest from white clover by selfing?

I tried to get seeds from white clover by self-fertilization but I could not get the seeds. As you know the white clover is generally fertilized by crossing. I wanna get the seeds by...

04 February 2021 9,152 4 View

What is the best method of preparing cell supernatant samples for a Luminex assay to detect cytokines ?

I am planning to run a Luminex assay using cell supernatant to assess cytokine secretion by tumor cells. I came across different methods in literature; some suggest seeding in complete media and...

03 February 2021 4,867 3 View