Kasturi Dutta .........This is a question usually discussed by university professors for two reason; (1) determining appropriate lab animals and number needed is a basic part of preparing a study and applying for funding. (2) because they’d understand your project best. I suggest a discussion with them. And include the following in your discussion. — There’s a general “concept” for determining number of lab animals needed, referred to as the “3 R’s”. The 3 links below will give you information.

1)__ Statistical Justification of Animal Numbers Used in Research and Teaching

By Dr. Shelly Naud, Research Biostatistician, COM Biostatistics Unit, UVM

https://www.uvm.edu/sites/default/files/Research-Protections-Office/Statistical_Justification_of_Animal_Numbers_Used_in_Research_and_Training.pdf

2)__ ANIMAL RESEARCH SAMPLE SIZE CALCULATION (AND CONSEQUENCES)

Philip Dubé, PhD — 2017

Taconic Biosciences

https://www.taconic.com/taconic-insights/quality/animal-research-sample-size-calculation.html

3)__ On determining sample size in experiments involving laboratory animals by Michael FW Festing — 2017

https://einsteinmed.org/uploadedFiles/administration/animal-care-use-committee/Lab%20Animal%20Article%20Re%20Determining%20Sample%20Size.pdf

Lee Clawson Thank you for your input