We do not have access to advanced instruments, therefore we are in need of simple methods such as Thin layer chromatography to identify compounds present in given plant extract by functional group. Kindly suggest, thanks in advance.
Hi, I attach the link of Camag TLC, I think you can search many things (also data bases) in this link, I hope you can find the methods that you need. Best regards
http://www.camag.com/en/tlc_hptlc/fields_of_application.cfm
Having only TLC is limiting, since it is more difficult to identify unknown compounds.
One can do functional group testing:
http://vlab.amrita.edu/?sub=2&brch=191&sim=345&cnt=1
Many TLC spray reagents are useful at detecting various specific functional groups:
http://lcso.epfl.ch/files/content/sites/lcso/files/load/TLC_Stains.pdf
You may need to run reference compounds to validate the identity of the compounds you find.
Looks like the Springer link didn't paste properly. Unfortunately, that was a Google search and I can't find it again. I apologize for the inconvenience. I deleted the broken link in my response above.
This link provides a little of the chemistry behind the spray reagents, helpful in understanding why they work and the functional groups that can be detected:
http://www.chemistry.mcmaster.ca/adronov/resources/Stains_for_Developing_TLC_Plates.pdf
We have the same problem in our labor. Some specific reageants hare helpful to identifie functional groups The book of Egon Stahl and Werner Schild i usefull for TLC of plant derivatives
May I know which functional groups are present in your plant extract? This would help to suggest anything further.
In our labor we extract diferents plants with different solvent and some time we try to identifie the contain of OH, CO or coumpound with N.we nalyse for exemple Carica papaya, mango, lemonsgrass, Detarium, Lantana Camara and different other local medicinal plants like Ficus sp, Curcuma longa,. I can send you if you want à copy of manual what we make in Würzburg for african plants geting information of the chemicals contents of these plants by using TLC with standard..
You can get better & authentic idea for identification of functional group by using IR or FTIR.
I agree with the answer IR or FTIR is the best way to identifiy the functional groups present in the plant extracts
Keita Yango Sir it would be a great help if you send the method you follow. Thank you
We do not have access to IR or FTIR, so we are looking for more simpler methods
Calculate the Rf values of your compounds than compare your results by already reported values of Rf in the literature with respect to the solvent system or mobile phase which you have used. Spray TLC with reagent like ceric sulphate or any other which is available than place the TLC on hot plate. Before doing this,Try to observe the spot in UV lamp.
Hello
I think you can use the phytochemical screening to determine the different chemical families in your sample, you can go after a fractionnal extraction.
you will find attached an article
Article Phytochemical Screening, Anti-inflammatory Activity and Acut...
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