Can you suggest some plant transformation vectors that I can use for the expression of GFP/YFP-tagged protein of my interest?

I want to generate stable Arabidopsis transgenic lines expressing my protein of interest fused with GFP/YFP under the control of its native promoter. I want this for localization studies. Please...

23 January 2021 2,790 3 View

Does cleaning up cDNA after reverse transcription reduce late cycle background?

I've been wondering if removing the random primers from the reverse transcription reaction either with a column or precipitation before using it as a qPCR template reduces the background a bit in...

25 December 2020 6,258 4 View

How to find porosity of a sintered ceramic compact ?

I want to know the porosity of my sintered ceramic composite ? Please help me out

21 December 2020 4,343 6 View

Precipitation of Pcl from polymeric solution ?

We working on PCL nano particles 1).Ketoprofen (10mg)+ dmso(5ml) Pcl (10mg)+ DCM(5ml) 2).Water 10 ml+ 10 mg When we mix both 1+2 after solublization (400 rpm) Why precipitation of polymer occurs?

09 December 2020 5,033 11 View

Can I include multiple outcome measures of the same construct, from the same study, in a meta-analysis?

I am carrying out a meta-analysis that looks at cognitive inhibition as an outcome. A wide range of assessment types can be used for this outcome and sometimes a study will include two or three...

15 November 2020 7,055 1 View

How can we confirm the formation of solid solution by using EDX point analysis and elemental mapping ?

I fabricated a composite say AB and both are carbides ceramics having formula AC and BC. I did EDX point analysis and observed A (45 wt %) and B (28 wt %), Carbon (26 wt %) and Oxygen (1 wt %)....

20 October 2020 7,419 7 View

Docking Approach for Combinational Drugs?

Docking studies are simple when it is a one ligand-one protein approach. However, what is the protocol for studying the combinational effect of 2 drug molecules on a particular protein, where we...

02 October 2020 8,009 22 View

How do you use the span and wagner equation of state (EOS) to calculate the mass of CO2 in a given volume of cylinder?

I am trying to use the equation of state of pure CO2 to calculate the required mass of co2 that could be contained in a given volume of cylinder.

24 September 2020 5,581 2 View

Pooling multiple continuous variables from the same study?

I am looking to meta-analyze the differential effects of physical abuse and neglect on depression in a meta-analysis. If I come across a study the reports a correlation between a continuous...

24 September 2020 4,211 3 View

How to calculate sample size for 3 way Mixed Between Within Subjects ANOVA?

Hi all, I have been trying to figure out a way to calculate a priori sample size for a mixed anova. I have a study that uses 2 x 7 x 2. The last IV is within subjects. I appreciate any help....

23 September 2020 3,635 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Is anyone working on a project focused on immigrants/newcomers working in the hospitality industry and how they have been impacted by COVID?

The on-going Covid-19 pandemic has had devastating economic effects on many industries. However, few have suffered to the same extent as full-service restaurants. Numerous studies have shown that...

24 February 2021 9,496 4 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

What is the role of work experience in motivating high tech entrepreneurship in Aviation industry?

Related work experience plays a vital role in motivating the Entrepreneurship. But when we talk about high tech entrepreneurship in Aviation industry, we talk about very high investment and high...

22 February 2021 376 5 View

Can anyone suggest a topic for the Impact of AI and ML in the financial institutions?

I am currently doing my Master Thesis in FinTech and I am thinking of doing my project on the impact of AI and ML on the financial industry but as the impact of these technologies on the industry...

21 February 2021 7,128 3 View

How do you obtain ASI (Annual Survey of Industries) plant identifiers, to create a panel data?

Dear all, Is there anybody who has worked with ASI (Annual Survey of Industries) Firm-Level data? I have extracted the data files with STATA, and now I need help with creating a panel for...

11 February 2021 876 4 View

Examples of GAMS syntax for a permutation flowshop scheduling problem?

Hi everyone, I am new to the field of industrial engineering. I need to formulate a gams model for a permutation flowshop scheduling problem. I will appreciate if someone can send me a code...

09 February 2021 891 2 View

How does data science impact business?

Data science is valuable to any business in any industry. From statistics and insights across workflows and recruitment and HR to helping senior employees make better-informed decisions, etc. Its...

08 February 2021 5,807 11 View

How to create a Hypothesis and Thesis in a statement?

03 February 2021 4,496 3 View

What's the role of bioinformatics in poultry science?

bioinformatics has a big importance in human science and lots are seeking to study that field, so it attracts me to know it's relation with poultry science and how we can benefit from it in...

20 January 2021 6,963 3 View