How to do interdisciplinary research combining literature and visual arts?

I am a first-year PhD student who would like to hear about other researchers' experience related to interdisciplinarity within the humanities, especially those combining both text and visual...

26 May 2020 2,889 37 View

What is the best method to store sectioned FFPE slides for long term storage?

I will be having FFPE blocks cut onto slides, I would like to know what would be the best method to store slides for long term storage. I don't want the slides to go bad. I am worried about...

16 January 2019 9,208 2 View

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28 September 2018 7,258 4 View

What are good control tissues for a MDSC Immunohistochemistry panel?

Hi Everyone, I am currently working on a human MDSC immunohistochemistry panel using CD33, CD11b and HLA-DR. I need to find good control tissue. I tried to use normal brain and spleen tissue...

23 September 2018 4,882 3 View

What are good control tissues for a MDSC Immunohistochemistry panel?

Hi Everyone, I am currently working on a human MDSC immunohistochemistry panel using CD33, CD11b and HLA-DR. I need to find good control tissue. I tried to use normal brain and spleen tissue...

08 September 2018 7,464 3 View

Can anyone recommend a sweetener that increases salivary secretion without disturbing original blood glucose levels?

To collect salivary samples (about 1 ml) from spider monkeys. The samples will be analyzed to determine cortisol, melatonin, serotonin and testosterone through ELISA tests. Thanks for any...

06 July 2018 3,605 3 View

Is it possible to detect serotonin levels in salivary samples through ELISA tests?

The technique is considered for a project to study physiological circadian patterns in free ranging spider monkeys, and thus the best suited sample source is saliva, because of its availability...

11 December 2017 5,978 2 View

Does anyone know how to calculate the number of peptide bonds of a protein after hydrolysis?

Need that calculation to obtain the degree of hydrolysis.

07 August 2017 6,463 0 View

Which ANTS/DPX concentration should I use to see solute leakage from liposomes?

I want to see how ANTS fluorescence in liposomes changes when adding triton.My idea is to encapsulate ANTS and its quencher DPX in the same liposome so that when adding triton fluorescence signal...

27 July 2017 9,640 1 View

How can I avoid nanoerythrosomes aggregation?

15 March 2017 10,022 6 View

In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein?

Hi In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein? I got this error in the add-ion stage: Fatal error "./ amber99sb-ildn-HHH.ff / force...

01 March 2021 8,747 4 View

Why does the sum of reaction forces not match the force component of a Free Body Cut in Abaqus?

I am using Abaqus to simulate a plate with in-plane loading. The boundary conditions allow the right edge to move up and down, the displacements on the left edge are fixed. There is no rotation...

23 February 2021 711 3 View

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23 February 2021 4,014 3 View

How to include additional force field in GROMACS?

I have a cellulose model system that is best described by the GLYCAM06 force field according to the literature. However, the set of force fields included in the GROMACS package doesn't include the...

20 February 2021 3,309 3 View

I want to simulate a system containing protein and heavy metals; which force field is most reliable for this purpose?

The protein I want to simulate has multiple heavy metals. I wanted to know which force-field I should use with GROMACS to achieve this. Most importantly, do the latest updates in the force-field...

16 February 2021 2,420 1 View

Why is there violent fluctuation of ThT signal in Amyloid beta aggregation?

Dear all, I am currently working on amyloid beta aggregation. In ThT monitered aggregation assay, I have already obeserved an increase in ThT signal, indicating the aggregation of amyloid beta....

15 February 2021 4,852 2 View

How to obtain parameters to run a simulation for benzene using CHARMM forcefield in GROMACS?

I have been reading this paper Soft Matter, 2017, 13, 1904 and in this paper, they simulate benzene in GROMACS using CHARMM. They mention that they performed simulations of benzene using the...

15 February 2021 5,561 3 View

Gromacs force field and water model recommended for protein hydrophobic study?

Dear All, I received a comment from reviewer about asking me selection of force field and water model. I choose OPLS-AAL and 3-point water model. They mentioned the combination of OPLS-AAL and SPC...

13 February 2021 1,364 3 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

Can anyone please suggest where I can find a reference for ReaxFF parameters for PTFE(Teflon) suitable for use in LAMMPS?

Trying to simulate a ice-PTFE interaction and am unable to find a reference which actually lists out forcefield parameters for PTFE-PTFE interactions using ReaxFF or even other force fields like...

08 February 2021 9,373 2 View