Dear all,
I am attempting to isolate RNA from Streptococcus mutans. Can anyone suggest me a protocol without using commercial kit from companies? Thank you all
Hi.
Long time ago I was successful with this method:
https://www.sciencedirect.com/science/article/pii/S016770120200218X?via%3Dihub
You should try it
Hi @ Miguel Fernández-Niño . Thank you so much for your answer. Did you use that protocol for Streptococcus mutans or other Gr+ bacteria?
I've had great luck with the Qiagen DNA extraction kits and including the extra steps for gram positive bacteria.
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
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