Which route of inoculation should be followed to check the activity of the compound against virus which doesn't invade the CNS by intraparetonial route, along with 1% starch intracerebral (ic) route (but the encephalitic virus does cause infection by ic route)? It would be appropriate to check the efficacy of the ic route of drug (compound) inoculation after pre-exposure of the virus by ic route in swiss albino mice.
Which route and method of administration of the drug should be done to check its antiviral activity?
If I understand you correctly, you are infecting ic and want to know if ic delivery of compound is a suitable treatment route. If you just want a proof of principle that the compound can inhibit the virus, this would be fine. You may want to determine the toxicity of the compound when administered ic to find a suitable dose before conducting antiviral studies to avoid unnecessary animal use.
You could also check the efficacy after ip or iv administration to check the passage of the compound across the BBB, which would be more suitable for use in a clinical setting.
Find your query at following links
AMM
http://www.kdheks.gov/epi/Investigation_Guidelines/Arboviral_Diseases_Investigation_Guideline.pdf
http://www.fda.gov/OHRMS/DOCKETS/98fr/05d-0183-gdl0002-01.pdf
http://www.doh.state.fl.us/disease_ctrl/epi/surv/CaseDefinitions/Acute.pdf
http://new.dhh.louisiana.gov/assets/oph/Center-PHCH/Center-CH/infectious-epi/Annuals/LaIDAnnual_EncephalitisWNV.pdf
http://www.odh.ohio.gov/pdf/idcm/wnv.pdf
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