My hygroscopic hydroalcoholic plant extract could not be chromatographed by using silica gel as the stationary phase. Only sticky complex mixtures were obtained.
Dear Uma Chandur,
What is needed is more specific information!
Are you requesting for a 'preparative' (column) chromatography technique?
I assume it to be. If that is the case, redissolve your sticky extract in small volume of methanol and then add coarse (say 50 mesh) silica gel and use a vaccuum-rotary eavaporator to remove the solvent. When it is still wet, remove the extract coated silica and dry it in a petri dish in a vaccuum desicator. Once it is completely dry, grind the clumps a fine powder and use it to pack on top of the column. It is like a 'sample' silica gel that you can use for chromatography using solvents of increasing polarity
Good luck
Sticky extract in plants is due to polysacchrides. Use resinssuch as HP-20diaion, XADs followed by Silical and finally by C-18.
Thank you so much dr suresh govindaraghavan.this was exactly what i was upto.preparative column chromatography.i shall follow your instructions.
thank you so much dr Robin joshi.i will consider the polysaccharide aspect also. right now im into preparative chromatography.I will definetily need your valuable advice while going in for standardization of the extract using hplc.
The easiest way is to pre-adsorb your extract onto silica by mixing a solution in the appropriate solvent with silica, evaporation and drying of the powder, and placing it on top of the column.
If your sample hygroscopic or high polar extract, it is better you will try with C-18 sillica gel with methanol and water ( formic or acetic acid buffer)mobile phase then those collected fraction try with sephadex column. i think this two chromatography system will give better results for polar compounds.
all the best!!!
Dear uma
Pl. check the attached publication for possible answer to your question.
Good luck.
Thank you very much sir.This book is an invaluable addition to my library. I will keep in touch with you
Dear Uma
I am happy the information served its purpose. I will be more happy if you can share it with other colleagues in your University working on the subjects detailed in the publication.
All the best for all your endeavors.
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