I want to know the recyclability of immobilized lipase that has been prepared. It is immobilized on synthetic polymer.
At first, the optimal pH and temperature of enzyme activity must be determined. For pH stability, enzyme solution was pre-incubated at different pH in the range of 3.0–9.0 for 30 min at optimum temperature for enzyme. The residual enzyme activity was determined.
Read the attached papers
Article Reduction of raffinose oligosaccharides in chickpea (Cicer a...
Article Immobilization of invertase on Celite and on polyacrylamide ...
Article Characteristics and stability of free and immobilized an Alp...
Hi Trupti,As Mr.Esam said you should know optimum condition for immobilized enzyme,after that you can measure stability against time,temperature and pH for it.
for temperature stability you should incubate immobilized enzyme(without substrate)in different temperature in optimum time and after that measure it' activity with substrate.
as same,for time stability you can incubate enzyme in optimum temperature and take it in different time, after that measure it's activity.
for pH as Esam said
best regard
What do u mean by operational stabilty? Do you mean recycling of immobilized enzyme.However, you may perform it batch wise or continously in optimal conditions.You have to check out the enzyme leach out every time.
You can read these publication on immobilization of lipase
https://www.researchgate.net/publication/289991956_Lipase_catalysis_in_organic_solvents_Advantages_and_applications
https://www.researchgate.net/publication/284078828_Cellulose_binding_domain_assisted_immobilization_of_lipase_GSlip-CBD_onto_cellulosic_nanogel_Characterization_and_application_in_organic_medium
https://www.researchgate.net/publication/298208671_Immobilization_of_a_novel_cold_active_esterase_onto_Fe3O4cellulose_nano-composite_enhances_catalytic_properties
https://www.researchgate.net/publication/298208863_Catalytic_Potential_of_a_Nitrocellulose_MembraneImmobilized_Lipase_in_Aqueous_and_Organic_Media
https://www.researchgate.net/publication/230745320_An_Innovative_Approach_to_Immobilize_Lipase_onto_Natural_Fiber_and_its_Application_for_the_Synthesis_of_2-Octyl_Ferulate_in_an_Organic_Medium
Article Lipase catalysis in organic solvents: Advantages and applications
Article Cellulose binding domain assisted immobilization of lipase (...
Article Immobilization of a novel cold active esterase onto Fe3O4∼ce...
Article Catalytic Potential of a Nitrocellulose MembraneImmobilized ...
Article An Innovative Approach to Immobilize Lipase onto Natural Fib...
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