you can contact me by e-mail if you want. sincerely yours, Christophe

I donthave any idea, Working about isolation and structure elucidation of lignans from plants

Lignin is localized by using various methods: 1) take sections of fixed or fresh plant tissue and treat it with phloroglucinol (1% in spirit) and allow it for 30 to 60 seconds. than add a drop of conc. HCL on it and put a coverslip. Lignin stains dark pink to red color. there are other methods to stain lignin monomers. (Johansen 1940, Plant microtechniques, Book)

Dear Vinay, Thanks but for lignan any chemical method is there?

as vinay patel has written take Phloroglucinol and HCL in equal amount it will take pink color

No histochemical test for localization of lignans in plant material is available so far.

Some common methods are Cross & Bevan (chlorine water-sodium sulphite) - distinguishes coniferyl alcohol and sinapyl alcohol derivatives - Brown and red colourations. Maule (1900) test (KMnO4+HCl+NH3) gives cherry-red colouration with syringyl lignins. Bucher (1968) method stains lignin dark brown and cellulose as light blue.

There are so many stains for ignin . The best quick result is add several drops of phloroglucinol to the section and then a drop of conc. HCL then draw off the excess liguid either observed directly or observe by adding glycerol 50% or chloral hydrate solution then cover with coverglass. Lignified elementwill turn crimson . Another stain is safranin it will turn pink and the excess stainns can be washed with acidified alcohol.Another stain is .05% AToludine blue O solution at pH 4.4 lignin stains greeish blue.- please try and reply

Lignan is different from lignin. Is it true that furfuraldehyde can be use for histochemical localization of lignan in plants? Thank you!

I'm working around the same question. Histochemical tests are available for lignin. Specific localisation of lignans would need some other kind of methods (e.g. TOF-SIMS, IR-microscopy, immunochemistry?). https://www.researchgate.net/profile/Christophe_Hano could you tell, what kind of methods you would suggest for specific lignan localisation (e.g. HMR2)?

Dear Piispanen,

Thanks. Not specific lignan, I did very basic studies on lignans Data (unpublished). Yes, as you said some other type of method will work for localization of lignans.

Thanks.