Can anyone share an electronic copy of the articles?

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Could you please suggest methods to compare free protein and immobolized protein binding properties?

I have an antibody binding generic protein and I need to compare its activity in a free and immobolized form. I understand that there are a number of methods to determine Kd value of a free...

05 August 2024 5,311 0 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

Do anybody knows any papers by W. M. K. Matsumura, N. M. Balzaretti, or R. Iannuzzi concerning chemistry of Spongiophyton compressions?

At the XIV International Palynological Congress and X International Organization of Palaeobotany Conference (Salvador, Brazil, 2016) W. M. K. Matsumura, N. M. Balzaretti, and R. Iannuzzi...

20 June 2024 10,096 2 View

Can I carbogenate and freeze sucrose-based ACSF a day or even a few days before an experiment?

I'm studying hippocampal pyramidal and granule cells in coronal mouse brain slices using patch-clamp. I use mixed NaCl/sucrose ACSF for cutting (ice-cold) and recovery (30 min at 35-37 °C), 0.5...

30 May 2024 3,472 1 View

Why are brain slices kept at near-physiological temperatures immediately after cutting?

Many protocols for obtaining acute brain slices for electrophysiology involve cutting the brain in an ice-cold solution, then transferring the brain slices into a solution pre-warmed to...

30 May 2024 2,056 2 View

Does storing brain slices in sucrose-based ACSF vs. normal ACSF affect slice quality?

I've recently started to patch dentate gyrus granule cells in coronal mouse brain slices (300 μm). I'm using mixed NaCl/sucrose ACSF for cutting (ice-cold) and recovery (30 min at 35-37°C) and...

27 May 2024 3,688 1 View

Is the apparatus of mathematical physics applicable to intelligence and its evolution?

Mathematical Physics of Intelligence and its Evolution We tried to start the development of this topic, but given the large number of mysteries related to the mind, its appearance and evolution -...

25 May 2024 2,822 2 View

Do you use simulation modeling of intellectual work? Do you test work flow and team building hypotheses using models?

I face problems with hypothesis testing on real teams in project management. Choice of methodology, composition and number of participants, etc. It takes 2-3 weeks to switch to a new process,...

03 May 2024 9,429 0 View

Polynomials of what type can be identically transformed into equation of an n-dimensional surface/curve?

22 April 2024 7,940 1 View

Protein molecular weight appears smaller on SDS-PAGE than expected, anyone knows the reason?

I am working on expression and purification of one cytoplasmic protein with His tag, in E coli Bl21(DE3) host cell. Here is SDS picture. Line 2 and 9 are cell lysate, 3,4,7,8 wash steps and 5,6...

20 March 2024 6,450 5 View

Do you think in which age of geology laterites can be more than other ages in Iran?

I want to know more about laterites in Iran.

31 July 2024 8,219 2 View

With attention to past my experiences on field geology I think any mineral ores can be in any geology area. Are you agree with me?

I want to know more about ore deposits in world.

27 July 2024 3,827 4 View

To now I have understood any mineral deposits can be in any tectonic setting environment. Are you agree with me?

I have 28 years experience in field works on economic geology exploration works.

18 July 2024 1,268 2 View

Do you think in which age of geology can be good komatiites in surface of the earth?

I want to know more about komatiites.

17 July 2024 2,870 3 View

Airborne Geophysics in Mineral Exploration ?

Appreciate your advice as I am asking about Airborne Magnetic Survey for mineral exploration (Gold and Associated Elements). What's the maximum depth for detecting subsurface extension and what...

02 July 2024 6,179 5 View

Geology Doctorate. Available for Post-doc and guest faculty. Please give suggestions. Anywhere in world ?

Geology Doctorate. Available for Post-doc and guest faculty. Please give suggestions. Anywhere in world.

09 June 2024 1,805 4 View

Do you think can be any carbonatitic igneous rocks in central of iran?

I think it is possible to be carbonatites in central of Iran with attention of its past tectonic and geology.

11 May 2024 9,523 3 View

Can dating detrital zircon in thin sections affect the representativeness of dates?

Dear Geology Community, I am currently researching the dating of detrital zircon in metasediments. Due to the small size of my samples, traditional mineral separation methods may not yield a...

09 May 2024 337 7 View

Help me in looking Supervisor from Germany?

I am currently working towards my PhD at Addis Ababa University in the field of Applied Geophysics, with a focus on utilizing potential field data to analyze subsurface structures and their impact...

28 April 2024 4,349 0 View

What is some background research on geophysical exploration technology and method?

23 April 2024 3,091 3 View

Alexey N. Morozov

Thank you very much! You helped me a lot.

Qiaoyun Hu

That's is a good question, thanks for reminding me about this issue.

The paper I uploaded is from AGU's EOS transaction, which is mostly of free-access, but some very early volumes are not. The paper I took is not with free access.

So.... I deleted the link. Sorry for my mistake.