I want to test an idea I have about fast purification and fractionation of different forms of peroxidases from potato tubers and roots and I am looking for sensitive and convenient assays.
At the moment I am using TMB for blots (Pierce) and home make TMB for activity in solution.
Recently I have tried luminol and seems it may work, however I need to optimise the assay.
I have been setting up the tube furnace to work with inert atmosphere, i.e. H2 and N2 in order to anneal the iron-based metallic powders. The furnace is equipped with vacuum pump as well. The...
18 December 2020 4,006 2 View
Dears, what is the best way to add any kind of noise (not only Gaussian noise) to 2D signal (image) for a given SNR? Do you know a good code in Matlab?
30 September 2020 8,788 2 View
Dears, could you recommend the papers in which the types of noise in MRI images were analyzed?
05 September 2020 2,810 4 View
Hi, I'm aware of numerous Explainable AI (XAI) solutions for CNNs, however they ~all seem to be tailored for classification (or segmentation). I also see that for a small home-made model (lets...
31 March 2020 5,082 5 View
Most of us experience far-reaching restrictions on private and public life, introduced by governments all over the world because of the coronavirus epidemic. Generally, these restrictions make a...
22 March 2020 6,992 95 View
Hi, Under 9 CFR 430, establishments in Alternative 2b and 3 are required to sample food contact surfaces for Listeria monocytogenes or an indicator organism....
10 March 2020 6,762 4 View
Should I perform such processing step before any analysis i.e. by using pseudo-invariant features (PIFs)?
30 January 2020 6,549 2 View
Assuming that particle consists of a photon that moves circularly in the loop, creating standing wave obeying the rule that its closed path 2*Pi*R=n*Lambda (1) (resonator where resonator length...
15 October 2019 8,759 12 View
What criteria can be applied to define friendship? Is friendship an object of scholars'/philosophers' thinking? And what does it mean that someone is someone's friend? What are the qualities of...
02 August 2019 6,716 24 View
Is it necessary to accept the idea of God established by a given religion to believe in the existence of God? Does belief in the existence of God inevitably involve the adoption of an ethical...
31 July 2019 4,599 97 View
02 March 2021 3,060 3 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
What do you know about biotechnology art?
02 March 2021 1,653 3 View
I am searching for a good place for the Post Doc,
02 March 2021 4,053 3 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?
25 February 2021 9,761 1 View
24 February 2021 1,365 3 View