Can anyone recommend the best assay for general peroxidases activity in solutions, in gels and on the blots?

More Krzysztof Treder's questions See All

How to add the name of journal?

How can I add the name of journal where I have published my article ? This name of journal is: Wolni i Solidarni. Między Ideą a Praktyką.

27 April 2024 8,705 0 View

How should I interpret the points which plot above the LMWL on the d2H vs d18O bivariate plot?

So, I am unsure how I should interpret the situation when the majority (let’s suppose 3/4) of my data aligns above the LMWL, while a smaller part (suppose 1/4) aligns below the LMWL. I conduct...

03 April 2024 1,958 0 View

Can you recomand any good neural stem cell lines (rat, mouse or human)?

just line, company etc

13 November 2023 3,618 1 View

Are you interested in a conference celebrating the centenary of Forster's A Passage to India?

The International E. M. Forster Society invites you to take part in a conference celebrating the centenary of the publication of A Passage to India. The conference will take place in June 2024 in...

13 July 2023 4,280 19 View

What could cause Western Blot signals to suddenly start getting weaker with each trial?

Hello, We are currently measuring concentrations of protein kinase A (PKA) and its phosphorylated from (p-PKA) in sub regions of the hippocampus using α-tubulin as loading control. We are using...

24 April 2023 6,421 0 View

What is the most complex model?

Can you identify the most complex model? How many variables in the model make the model structure feasible?

12 March 2023 196 4 View

How can I compute the area between unique regions in a FEM model with tetrahedral elements?

Hello, I have a finite element method model comprised of tetrahedral elements. The model has a few million elements and a few hundred unique regions. I need to compute the statistics of i) which...

30 June 2022 8,125 2 View

Is a gradient mesh size possible in gmsh with .stl files without using a "Threshold" field?

Hello, I am creating polycrystalline microstructures for crystal plasticity FEM simulations. I use the DREAM.3D software to export .stl files that are then meshed using gmsh. This works well and...

20 December 2021 1,610 4 View

What is the best assay to measure viability of human macrophages?

Hello, I want to investigate the influence of certain drugs on the viability of human bone-marrow derived macrophages cultured in vitro. What viability assay do you find to be most suitable for...

26 April 2021 1,662 5 View

Where can I find MRI datasets with real images corrupted by heavy Rician noise?

I designed an algorithm for MRI data denoising which has good properties under heavy Rician noise (sigma is greater than 80). The method was tested on Brainweb's phantom and Matlab's ricernd()...

14 March 2021 1,829 4 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

What exactly is it about biotechnology and medical research?

I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together

28 July 2024 3,798 4 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Can anyone help me with in providing the procedure for purification of GSTtagged protein by gst affinity chromatography without an automated machine?

Purification of gst tagged protein

25 July 2024 1,720 0 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

Dear fellow researchers studying in molecular biology, are there any tips on how to avoid plasmid dimerization when extracting plasmids?

Using INTRON's DNA-spin Plasmid DNA purification kit to extract plasmids from specific E.Coli strains, my team and I have encountered numerous plasmid dimerizations that lead to over-weighted...

23 July 2024 3,490 6 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View