Can anyone recommend a special preparation of mobile phase to estimate levels of various monoamine neurotransmitters on HPLC-ECD in the same assay?

11 February 2015 1 5K Report

Do you recommend a special preparation of mobile phase for estimate the level of various monoamine neurotransmitters and metabolites on HPLC-ECD all in the same assay? and do you recommend any special column? I need to find DA, DOPAC, NE, HVA, 5-HT, 5-HIAA levels on just 20ul of Dialysate (comming from mycrodialysis). I have a particular concern with pH levels because my columns doesn't work well on very low pH's (2.65 or lower).

Thanks a lot in advance.

Awanish Mishra

I have reported a method in Mishra and Goel, 2013 Neurochemical Research. you can try that.. this provide suitable pH range for you. But I havnt included 5HIAA, DOPAC, DVA in that. Soon our research group will publish the method for estimation of same using HPLC-ECD.

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