Do you recommend a special preparation of mobile phase for estimate the level of various monoamine neurotransmitters and metabolites on HPLC-ECD all in the same assay? and do you recommend any special column? I need to find DA, DOPAC, NE, HVA, 5-HT, 5-HIAA levels on just 20ul of Dialysate (comming from mycrodialysis). I have a particular concern with pH levels because my columns doesn't work well on very low pH's (2.65 or lower).
Thanks a lot in advance.