How to extract pure form of protein from either powdered venomous saliva or fresh venomous saliva milked from reduviid predators?
It would seem more logical to extract pure protein from a liquid then a powder. You would first have to find a model of a pure protein and its chemical and molecular formula. Then it would be wise to use some type of microscopic equipment to make sure that there is a pure protein in venom. Once that it is determined that pure protein is in the substance that you are testing for, I would think manually injiecting the protein out or what you are looking for as you are looking under the microscope.
Dear Ruth Taylor-Smith, we already experimented and analysed the venom with SDS-PAGE and also MALDI-TOF. Results are says both the liquid venom and powdered venom contained protein. However, analysed says some impurities are in the milked venom.
A milking process is kind of rigourous. You say the results signify signs of protein. I beilive that when you figure out the molecular formula you can find substance that is compatible to a extract it using another element. Many elements are connected through a molecular combination. It is like a magnet. If you drop a magnet into a combination of alkali and metal substance it would probably cling to metal. The must be a substant that pure protein will cling to for extraction. Unless disintegrate the flesh of the animal.
You are quite welcomed. I really try to think logically with the ample of information given. I start thinking that particularly meat is considered a pure form of energy and meat is considered a protein.
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