Can anyone provide me with plasmids pUC18-RedS or pRKaraRed?

15 July 2016 0 2K Report

I want to knock-out NRPS operon (or its part) in our Pseudomonas fluorescens strain.

Unfortunately, the literature on generating knock-out mutants of P. fluorescens is quite poor. Therefore I decided to try with simple pUC18-RedS or pRKaraRed protocols, which were previously tested for e.g. P. aeruginosa and P. putida.

I would be very grateful if anyone could provide me with one of these plasmids (pUC18-RedS or pRKaraRed).

Or maybe do you know any other protocols, that I can use for P. fluorescens?

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