Can anyone please suggest me how can i adjust the ratio of mobile phase solvents in HPLC analysis of Insulin?

09 March 2022 1 3K Report

I have used insulin vials for the HPLC estimation. Acetonitrile and Orthophosphoric acid were used in 50:50 ratios. What modifications should I use in the composition of mobile phase solvents to estimate insulin powder? Insulin vials contain meta cresol as a preservative and WFI as the solvent.

Michal Kriegelstein

What is your issue? Are you not getting separation of the compounds or you dont see any in your chromatogram?

We use 30% B -> 32.5%B in 60 min. (A=0.1% TFA/water , B=0.1%TFA/ACN) on Nucleosil C18 for insulin separations. But ofc, we separate from other compounds than cresol(s)

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