The classical technique is to use a close container and allow the CO2 to absorb into a known quantity of strong base, e.g. KOH. After a period the container is opened and the excess base is titrated using strong acid. Modern techniques use continuous gas sampling/recyling coupled with (gas) analysis using IR. This obviously provides more data and avoids the problem of having either too little or too much consumption of O2 in the container.
Thank you Dr. Paul Milham, I got the procedure but after titration,I need to do some calculation to know the CO2 evolution. That calculation steps I could not able to find from any articles.
Dear Praobhu Mani - if you go to my Profle you will see a document called ' Laboratory Manual of the Soil Microbial Biomass Group, Rothamsted Research ', which on pp. 50-53 contains the information you're seeking, for a given method of collecting CO2 that is. It also gives details of the orignnal reference - Tinsley et al 1951, which would be useful, if proabably difficult, for you to get hold of.
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