Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Please. Could you help be in implementing Fractal Discrete Cosine Transform algorithm in Matlab?

I do need the Matlab code of Fractal Discrete Cosine Transform (FDCT). Can anyone who has already implement this code, help me with the implementation of this transform?

24 February 2021 5,602 2 View

What is the reason film thickness increased?

what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....

17 February 2021 8,987 5 View

Queries regarding the rain flow counting algorithm?

02 February 2021 9,790 2 View

Electroactive surface area of bare and the synthesized material?

For electrochemical sensors, the electroactive surface area (ESA) show higher value for the working material as compared to bare (reported in several papers). My bare electrode is FTO and working...

17 January 2021 7,220 3 View

How to determine the genetic potential of the genotype in terms of fruit size and weight?

In addition to genetic and environmental factors, fruit size is also influenced by crop load, rootstock, orchard planting system/tree training system, pollination, water and nutrient management,...

13 January 2021 9,309 4 View

Is it possible to ligate two restriction ends those have no complementary bases?

In my lab,we are trying to construct a control plasmid. So we have to remove the insert gene which has two restriction ends:one is XhoI and other one is Hind III.So after double digestion we want...

01 January 2021 3,271 4 View

Is it machine learning models that have replicability capability?

We are doing the prediction of soil nutrients using spectral information by applying machine learning models. More than 300 soil samples were used from one district for analysis. We got the...

21 December 2020 9,663 3 View

How can I analyse and compare crop yield under different irrigation methods?

I have to compare yield parameters of a tree crop grown under three irrigation methods. But these systems are already established separately in three adjacent fields. Since I can not follow a RCBD...

21 December 2020 3,065 5 View

How can i select suitable gene sequence at Ring stage of Plasmodium Falciparum for primer design?

I am new to PCR. We are working on PCR based amplification of targeted DNA sequence of Plasmodium Falciparum. For that, We are targeting the Ring stage of the parasite. I want to know on which...

15 December 2020 6,954 3 View

Any references about insects or animals that can damage or weathering of rock or fossil material?

Are there any references to insects or animals that can cause damage or weathering to the rock or fossil material? books or paper that would be nice. Thank you very much!

24 February 2021 6,805 3 View

Since I am design automatized grader for forest seeds, it is important for me to know the following?

1. Is there a difference between a dormant seed, for example, of Scots pine, a fossilized dead seed, and an active live one by spectrometric or other characteristics? 2. Is it possible to...

26 November 2020 9,275 2 View

Which are the drawbacks of using a medical CT for scanning fossils?

I am in the need of scanning some fossils for analyzing their inner structure. Unfortunately I don't have access to a proper CT or micro-CT of industrial use that suits nicely fossils and neither...

16 November 2020 1,748 6 View

Is there any report of commensalic/parasitic/symbiotic relationship between fossil communities from Indian examples?

I would like to collect and understand information on commensalic/parasitic/symbiotic interactions among fossil communities from Indian geological records. if there are any reports/publications in...

14 October 2020 6,253 1 View

Anybody can determine this micro-fossil?

Dear Researchers, I found it from Permian limestone strata in Julfa province (North-West of the Iran country). It should be mentioned, this microfossil extracted from samples collected of the...

29 March 2020 574 5 View

What is the best method to stop pyrite decomposition of a fossil?

The problematic specimen is a fossil endocast of a calamite stem from the Carboniferous. It contains some pyrite and the oxidation process is already running. What is the best method to stop the...

22 March 2020 9,246 19 View

Can somebody help me and recommend publications for extracting Radiolarian microfossils from the Middle to Upper Permian strata?

Dear researchers, I'll be grateful if it is possible to recommend any method papers for extracting Radiolarian microfossils from the Middle to Upper Permian. Sincerely yours, Soheil.

22 March 2020 5,895 4 View

What is the best way to date a phylogenomic tree?

What is the best way to date a phylogenomic tree using fossil calibration? It is more or less straightforward with a few Sanger loci using programs like BEAST, but it becomes intractable with...

26 February 2020 4,263 8 View

Can anyone suggest what is this microscopic fossil extracted from Middle to Upper Permian limestone strata?

Dear Researchers, I found it from Middle to Upper Permian limestone strata in Julfa NW Iran country. This microfossil extracted form sequences that belong to the Stratigraphy and bio-event...

16 February 2020 3,088 10 View

Phylogenetic analysis of morphological dataset adopting Bayiesian inference : which software?

Dear colleagues; I am approaching Bayiesian phylogenetic analysis, and I am dealing with (fossil) morphological datasets. I am especially interested in tip-dating and I would like to have an...

01 February 2020 1,097 7 View