Periodic acid cleaves the diols present in carbohydrates, leaving two aldehyde groups. The dye used in the reagent has amine groups which form a Schiff base conjugate with the newly formed dialdehyde, thus binding the dye to the oxidized carbohydrate.

Thank you so much  Masood Sepehrimanesh, i will look forward for this method and it looks easy then the SDS PAGE method.

Dear Greg Cote thank you for your explanation at least I get to how it works. It will be great if you can give me reference for the method. I have not find any suitable methods as everyone try to modified it.

Some of the major companies that supply biochemical reagents to laboratories can  supply reagents/kits for the fluorescent or colourimetric or enzymatic detection of glycoproteins in polyacrylamide gels and  also after blotting onto membranes. They also provide information on how the detection method works and detailed protocols for the use of the reagents. Search Google for < glycoprotein gel staining> . You will also get some references in PubMed that you can access for original methods articles and more information on the method.  On gels or blots you will need to include quantitative standard to generate a standard curve for accurate quantitation.

Thank you so much Peter Jackson for you valuable suggestions.