Can anyone help me with this question?

01 January 1970 0 8K Report

what is the most reasonable mechanism for hydrothiolation of terminal alkynes?

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How to clone the GOI into ROSA26 donor plasmid?

I am new to CRISPR Knock In. I want to clone a gene into ROSA 26 donor plasmid to Knock In a gene into the mouse genome. I have selected the addgene plasmid # 74285. But I cannot figure out where...

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Hello, I have a CRISPR related question. Can anyone help me?

I wanted to knock out MSH2 in mice cell line CH12F3 and after gRNA construction, transfection and single cell sorting, I got single colonies. I used two guide RNAs to give a deletion but when I...

04 May 2019 8,071 6 View

If I’m expressing a mammalian protein in bacteria, can I use an antibody for the protein that has species reactivity for human, for Immunobloting ?

for recombinant proteins do we have to use specific antibodies?

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How do I amplify a 7.4 KB fragment in mouse genomic DNA ?

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02 March 2019 1,401 4 View

How to design best primers to amplify a region in mice genomic DNA?

What is the database I should select under the Primer Pair specificity parameters in NCBI primer design tool?

02 March 2019 7,055 2 View

Has anyone tried using zeta probe membrane in DNA slot blotting?

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01 January 1970 2,877 0 View

Anyone familiar with DBCO -CY5?

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01 January 1970 280 5 View

Can anyone suggest me a method to analyse altered replication dynamics other than DNA fiber analysis?

One of the compounds that we synthesized in our lab can result in replication fork collapse. We have tested for gmma H2AX foci that shows the ds strand breaks. But we need to test for the...

01 January 1970 6,431 0 View

Can anyone help me with this Urea-PAGE gel issue?

When I run my 17mer DNA oligos on PAGE gel I can see a smear above my DNA band. Does anyone know a possible reason or how to minimize this? I have attached a gel image here as well. Thank you

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Can anyone suggest me a suitable solvent system for Cl and azide separation where both are linked to n-hydroxyphthalimide with an ethyl group?

Cl and azide linked to n-hydroxyphthalimide

01 January 1970 1,847 2 View

What is the reason for current dropping in OER , LSV curve?

I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...

10 August 2024 3,629 1 View

What may be the reasons for failures of Tube toi Tube Sheet Joints in Boiler Drum ?

We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.

08 August 2024 3,161 0 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Reason for discontinuities in my Band structure?

Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...

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Is it fare for editors of reputed Journals to put on hold manuscripts more than six months without review updates, Are they harassing researchers?

It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...

24 July 2024 8,596 2 View

Has anyone tried freeze flow samples in -80C freezer or liquid nitrogen before processing?

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My Cyclic voltammetry curve doesn't show cathodic peak of Cu2+ to Cu+. how to solve this problem?

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08 July 2024 2,388 4 View

Hello, I’m growing three species of microalgae under heterotrophic and mixotrophic conditions. Both the two turned yellow?

What could be the reason for mixotrophs to be yellow too instead of green? Thank you

08 July 2024 594 2 View

What are possible reasons for having the repeated wavy patter in Raman spectrum?

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Which river basin has lowest total replenishable groundwater resources &utilization of irrigation potential created lags behind potential created why?

Which river basin has the lowest total replenishable groundwater resources and utilization of irrigation potential created lags behind the potential created what are the reasons?

03 July 2024 9,638 0 View