CTAB-PAGE protocol .

If your sample has a total positive charge and you are running a native gel (no SDS) why not reverse the polarity of your gel ? i.e connect red lead to black input in power pack (remember to reverse them afterwards)

Here is one more protocol, there is also a protocol in "The Protein Protocols Handbook"

http://www.sciencedirect.com/science/article/pii/000326979290224U#

Hong Yu, your article doesn't show the protocol. :(

Ian Teo, the problem is that I want to have a line with a Weigth molecular line, and I need to cover with a positive detergents and not with SDS.

There were only a few papers published on CTAB PAGE. We used capillary electrophoresis format https://www.researchgate.net/publication/51675274_Size_separation_of_proteins_by_capillary_zone_electrophoresis_with_cationic_hitchhiking, Check the references for papers separating proteins in slab gels. For cytochrome c and other basic proteins you may need carbamylate your proteins https://www.researchgate.net/publication/51636639_Chemical_modification_of_proteins_to_improve_the_accuracy_of_their_relative_molecular_mass_determination_by_electrophoresis.   

Article Size separation of proteins by capillary zone electrophoresi...

Article Chemical modification of proteins to improve the accuracy of...