Hello work elucidating structures of natural products, can help you

Hello Pawan

If You send me your spectroscopic data for analysis, I can help you.

Sorry for dealy. Here giving ir and nmr data

C13 nmr

1h nmr

Working on data i reached the conclusion that compound contain anthracene nucles, 3 hydroxy, 1 methyl and one carboxylic group. I have followed the extraction procedure for glycoside and therefore compound can be anthroquinone glycoside. please help to confirm structure

Also adding gc-ms data

Hoping for quick reply

thanks

Dear Pawan Kaushik

I recognize the compound you send me the spectrum data as Quercetin, a yellow flavonoid common in plants.

The solvent used to do the spectra was a mixture of DMSO and CDCl3. The absorption peaks show little changes in relation to other literature data. At 300 MHz was not possible to see meta-coupling constants.

In the H-NMR spectrum, the peaks at 0.86 ppm and at 1.31 ppm represent CH2 groups of a long chain from a fatty impurity. The peak at 12.42 ppm is from a 5-OH group that forms H-bond with oxygen of carbonyl group.

A table attached displays the nmr data of quercetin and of your sample.

Thank you so much Mam@Selene Maia de Morais

Now its clear that my sample was found to be quercitin.

Please suggest me a way to publish this data

Thanks

Regards

Pawan Kaushik

Dear Pawan

To evaluate the possibility to publish your data I need more information about the plant like: Quercetin is the first time that the occurence is cited in the plant?

The plant has any medicinal use? Does quercetin could be reponsible for the activity of the plant?

There are many other informations required to justify of publishing a study of a known compound.

Prof. Selene M Morais

Respected Prof. Selene M Morais

1. I have already evaluated the extract for antihyperglycemic and antilipidemic activity from which i isolated this compound

2. Till now no one has reported this compound from the plant.

3. According to literature plant is used for antidiabetic activity

4. Moreover quercitin is also reported to be antidaibetic

I have already published papers on pharmacological activity of different plant on extract level but not on isolated constituents.

Now please suggest me a way out or send me a research paper so that i can prepare a manuscript according to it.

As it was my first time dealing in isolation, elucidation.

Regards

Pawan Kaushik

Respected Mam

I have one more query about the structure. i observed that 13 C NMR spectrum of compound which shows integration of the methyl carbon to the compound skeleton. moreover, the peak intensity of that signal in 1H NMR also confirms its contribution to the skeleton.Moreover if that signal would have due to fatty acid impurity then that might should lost in the noise of 13 C NMR as other impurity signal did. so you please can help me about the assignment of that signal.

Regards

Pawan

1HNMR is more sensitive then 13CNMR spectra and show every thing in the sample because it needs less amount to run the spectrum. The peak In 28.53 ppm in 13CNMR spectrum is not of a Methyl group, it is due to several CH2 carbons from the fatty material chain. If you ask in the chemistry department to do an experiment DEPT 135 in NMR spectrum you will know that the peak at 28.53 in 13CNMR is a CH2 group.

Thanks for your prompt reply. Now please let me know how to publish this, I have already given the details of work done.

Regards

Pawan

Hello Pawan Kaushik

Please find attached an example of a work with quercetin in a plant which displays antidiabetic activiy.

Beste regards,

Selene

Thanks mam