Smering in Pcr Amplification ?

I am facing a problem regarding smearing in PCR amplification since two months. But, before six months the results was there using the same primers at 67.2-degree celsius annealing temperature, 5%...

23 November 2020 4,394 5 View

Dear all, I am interested in a project relating to agricultural waste management. Is there anyone who is interested to collaborate?

In this project on agricultural waste management, we will discuss the problem of agricultural waste, how it can be managed, and its legal framework. Area of study: Distt Patiala and Ludhina,...

13 July 2020 8,112 4 View

Whom should I approach for analysis and assembly of NGS reads of nanobodies in the camel phage display library?

We constructed Nb-phage display library derived from an LPS-immunized Indian desert camel and have recently subjected it to NGS using Illumina MiSeqTM platform. The raw data of NGS reads was...

24 May 2020 8,054 3 View

I am getting error saying could not open conf.txt for reading in AutoDock Vina.?

When I tried to docking windows cmd shows Error: could not open "conf.txt" for reading. I have tried to change the path for cmd e.c.t, but it was not helping. And I tried to change from program...

10 May 2020 8,821 4 View

Determining encapsulation efficiency of Liposomes using Triton X-100??

I am trying to determine the encapsulation efficiency of liposomes using Triton-X 100 in order to disrupt the membrane followed by extraction of lipophilic drug using hexane..Whenever i put hexane...

30 January 2020 3,404 3 View

How to change the intracellular pH in human cell line?

Hi All, I want to study the effect of change in intracellular pH. Can you suggest, how can I change the intracellular pH in human cell line. Is there any specific cell line suitable for such...

17 July 2019 1,054 1 View

How many iterations are generally required to get convergence for PEMFC simulations in ANSYS FLUENT ?

14 February 2019 849 4 View

Where can i find PCG(Phonocardiogram) with ECG(Electrocardiogram) database?

I found some ECG along with PCG from Physionet 2016 challenge dataset with name "a". But, description of ECG is unknown hence i am unable to apply particular algorithm to detect important...

24 December 2018 1,017 5 View

Where can i find waveletScattering function used in matlab?

I am currently using Matlab 2018a while i search help for waveletscattering it shows "waveletScattering not found" . Or is it compitable only in new version i.e Matlab 2018b.

23 December 2018 3,665 4 View

What is the difference between Bulk Metallic Glass and High Entropy Alloy?

What is the Difference between Bulk Metallic Glass (BMG) and High Entropy Alloy (HEA) with respect to a specific parameters (no. of elements, Purity of constituting elements etc.). Also,...

11 December 2018 8,793 7 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How to approach CFD simulation results of a flow past a cylinder?

Hi all, I'm doing a transient simulation of flow past a rectangular cylinder close to the free surface. As far as I'm concerned, the common practice is to discard the initial stages of the...

25 February 2021 1,348 3 View

How to visualize stress in a coating defined by "Skin" in Abaqus?

Hello All! I'm trying to model a contact between a coated substrate and a tip in Abaqus. The coating is ultra-thin compared to the geometry of the substrate. So I defined the coating using the...

16 February 2021 1,594 3 View

How to perform Cox proportional hazard analysis or Kaplan-meier survival analysis in SPSS?

At first please see the attachment. This experiment was performed for a period of 7 days (n=100). After 7 days, we found different level of survival rate (%) for each treatment. Now, I want to...

15 February 2021 9,145 2 View

How to calculate visual angle of a visual stimulus?

I am currently trying to figure out how to calculate the visual angle of some visual stimuli I used in an experiment. I think I have all of the information I need to calculate this. One is a...

15 February 2021 3,189 2 View

Which DL architecture is better to work on handwritten data?

I am working in the area of Handwriting recognition for Bangla. As there are many DL architecture, I am confused which one to select.

15 February 2021 4,996 1 View

How to obtain regions around the person bounding boxes(excluded the detected people in scene)?

Hi all. I have a set of coordinates of bounding boxes for AVA Dataset. This dataset is used in action recognition. My goal is to get the areas around the people (people are inside boxes) and do...

13 February 2021 2,538 2 View

Hyperspectral Image : open, visualize, process in Python?

I am looking for a tutorial or course to learn how to open, visualize , process hyperspectral images in .hdr , .raw format using python. Any recommendation for a tutorial is appreciated ? Thank...

08 February 2021 7,896 3 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

How to convert a folder of images into h5 file?

Hello everyone, im currently working on a recognition system. I have a folder full of images and I need to convert the whole folder to .h5 file. this will help me with the next step in recognition...

01 February 2021 1,667 2 View