Hi Toumi,

We do protein purification by classical methods such as salting-out, gelfiltration and ion-exchange. We have the resin for all of these. Our laboratory is not well equiped but at least will enable us to do these techniques.

Its like what i have in my laboratory but i need to hplc.ms.ms and séquencer of protein

No, unfortunately, we do not have lc- ms.

What's your protein specificity? did you try classical methods to start with? I can try to help but I should have the capacity to do what you want. In my laboratory, in Sudan, we do all the purification steps, we also prepare the affinity matrices in the laboratory (we don't buy from any chemical company). At present we have:

Mannose-agarose

Guar gum

Galactose agarose

N-acetylgalactosamine

N-acetylglucosamine

Fetuin-agarose

In which all (except fetuin-agarose) prepared in our laboratory.

Regards

Emad

Thank you for your proposition about the affinity of my molecule is for Gal-Nac and lactose now i'm preparing for my felowship a italia , we can working on future in the same project good Luck