Collected from a stagnant freshwater pool, dark green color, mucilagenous. I am doubtful as to whether it is gonium, pudorina or eurodina? It is not in a colony, it's unicellular. Photos taken are in 100x oil immersion lens and 45 x & 10x view.
Minakshi: Could you provide full citations of the books you mentioned, please? And which chlorophytes you mean?
Euglenophyta, I guess.
May I know from where have you collected those?
The algae was collected from a very small artificial pond near our institute. Water remains stagnant in it.
Yes, it can be identified. for identification you need to send the culture to our lab.
Along with detailed observations at [email protected]. So that I will help you.
Is it a unicellular or filamentous algae, that will help in identification.
Thankyou Tandon Sir for your support.
Its a unicellular algae. The photos are of temporary slide preparations after we brought it to our lab from field. Culture is under process. Will Come back to you soon with it.
Infact as a teacher, I want to under go some good algal culture training program.
Basically I am a zoologist, but as I teach aquaculture biotechnology, I come across algae, since last 2 years, and has developed interest now.
Mr. Hauer,
Yes, it did form neustonic film in that pool. I can say, it looked as if it formed a film of some oil spill. The film was very thin on the water surface.
Then it is most probably some Euglenophyte. I can not tell you any name, because the cells on your photographs are probably dead or almost dead. For more precise identification you need living (usually moving) cells with at least visible cell shape and surface.
Yes Mr. Hauer, I found along with these lot of moving euglena. No other algae was present in it. I could not take good photos due to limitations of microscope at my end.
It looks like a Haematococcus sp. to me. Did they form reddish-brown blooms during day time?
Your unknown organisms are euglenophytes, most likely Euglena but you cannot really say for sure when they are in this form. When stressed many euglenophytes will "ball-up" and become embedded in mucilage.
We at Dr.D.y.Patil Vidyapeeth(DPU) are working and collecting algal culturesfrom different zones of India. We have launched a algae data base, you can refer on
http://algaedb.dpu.edu.in. You may get some help from this database.
It looks like an encysted Euglenophyte (probably Euglena). If so,paramilon grains seems to be arranged into diplopyrenoids. Take a look on swimming specimens. Good luck!
Try to get a culture of the algae. Regarding the recipe, see http://web.biosci.utexas.edu/utex/mediaDetail.aspx?mediaID=61
We are in touch
Regarding the possibility that it were Haematococcus or an euglenphycean, just try staining with iodine solution.
If U see the first photograph, some astaxanthin is found which takes red colouration to the cells. Due to environmental pollution or man made pollution the pigment become degraded. So most probably it is Haematococcus. U try to culture this in your lab and check the condition of cells regularly. I appreciate Carlos and Lim.
Yes.. Even when identification on the basis of photos is really not an easy task, I insist with the "Euglena Theory", and agree with Marina. The red spot in one of the cells of the second micrograph, looks like (almost not doubt to me) the conspicuous stigma of E.. Nehal: move delicately and carefuly the micrometer at 1000 x and try to pinpoint the styriated pellicle of euglenoids. I am almost sure you´ll get it! Best to all!
Thanks a lot carlos, i too agree that it should be euglena, as in the field from where the sample was taken, no other moving organism was found except euglena.thanks a lot again.
it is so genial, in this site I can to learn too much. Sharing, teach and help are good for the research on the world.
Zohre, I think that most of us are able to help you. Please, share with us some photo or whatever the document you have that could help us to do so.
It appears that it is one of the chlorophycean algae that is difficult to identify at least from these photomicrographs. Therefore, more information is required: different stages of cell growth, as it may be cysts stage of Haematococcus with brownish cytoplasm, if so, transfer the cells to nitrogen depleted and or phosphate depleted medium and take photographs for astaxanthin accumulation if Haematococcus, of course in high light incubation. Also, Haematococcus grows actively in low light and complete medium as green bi-flagellated pear-shaped cells. Also cell diameter information will be helpful. It is also most likely to be Chlorococcum sp. if those tiny globular cells are daughter cells. Or these are gas vacuoles??
One point of interest: The presence of brownish to reddish pigmentation is not an argument to discriminate between Euglena and Haematococcus cysts. E. sanguinea (which also accumulates astaxanthin, as H.) is the best example of this confusion. See https://www.google.com.ar/search?q=euglena+sanguinea&noj=1&tbm=isch&tbo=u&source=univ&sa=X&ei=T7zNUbmWFujx0wHtiYCQCw&ved=0CC8QsAQ&biw=1280&bih=914
and links therein.
More: This taxonomic decision (Euglenophyte vs. Chlorophyte) is very easy to solve. Iodine (Lugol`s) solution test, as I stated above, is positive for starch (Viridiplantae universal intraplastidial reserve polysaccharide) , and negative for paramilon.
http://en.wikipedia.org/wiki/Iodine_test
http://stainsfile.info/StainsFile/stain/solutions/lugol-iodine.htm
Further discussion on this topic has no sense if this point is not solved.
we keep in touch.
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