I am working on the effects of methanogenic inhibitor on mcrA gene copy number abundance in the rice field. So I want to get a good gene copy number. How much ng of soil DNA template is used as optimum to get good gene copy number on qPCR?
Aarón Barraza
I recommend to carry out an standard curve of your gene cloned in a vector to establish the relationship between DNA amount and copy number. And for your soil DNA sample ensure to obtain between 10-100 ng for your qPCR analysis by the fact that most of the qPCR equipments have an optimal amplification range between those values of DNA concentration.
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