Can anyone explain why there are some shere silica?

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Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I use different force field for protein and ligand?

Hello, Dear! Generally, I used CHARMM36 + cgenff force field for simulaiton. But,, due to certain parts of lignad, force field cannot be generated using Cgenff. Can I use different force field...

10 July 2024 9,513 3 View

Ways to mitigate stimulation electrodes coming off?

I have a cathode electrode (5 x 5cm) on the femoral triangle and an anode electrode (5 x 10cm) on the gluteal fold. We are doing repeated high-intensity cycling followed by repeated Wingates. We...

07 July 2024 833 1 View

How to solve Gaussian Convergence failure?

To whom it may concern, I'm working on optimizing the molecule using gaussian 16. However, it is suffering from the problem of convergence failure. I attempted optimization several times, but...

30 May 2024 6,373 3 View

Setting chlorine injection concentration??

I want to experiment with microbial control through chlorine disinfection. What I have on hand is 5% sodium hypochlorite solution. I want to set the chlorine injection concentration to 1, 3, 5,...

01 May 2024 3,741 0 View

What is the mechanism of ionization of transition metal?

can anyone give me advise about charge transfer transition of transition metal and rare earth element? and usually people see the FL and absorption, emission spectra of those materials in glass,...

08 March 2024 7,916 1 View

How to dissociate clumping cell?

H1781 cells are shown to tend to clump each other. H1781 is adherent cell and detached by using trypsin-EDTA. however, over-digestion using trypsin might provoke cell clumping. they seemed like...

03 March 2024 7,757 4 View

What is wrong with the mitochondria isolation protocol?

Hello I am currently trying to set up a new protocol. Our lab is planning to perform study about mitochondria, and we still couldn't set up a proper protocol. The kit we used is from abcam...

05 November 2023 9,681 0 View

Why have most research studies related to crack branching been conducted only on amorphous materials?

I've been studying the crack branching and dynamic fracture of brittle materials, and I just noticed that most of the experiments are conducted using amorphous materials like PMMA. is there...

26 October 2023 7,536 0 View

M-wave acquisition Matlab script?

I would like to acquire EMG, torque/angle (from isokinetic dynamometer), and stimulation data via Matlab. I have a chassis system that is connected to my computer with Matlab. When the stimulation...

18 July 2023 7,425 2 View

What is the best conditions for formation Magnetite ore deposits?

I want to know more about Magnetite deposits.

06 August 2024 1,066 0 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

Short Synthesis of Graphene Oxide from Natural Graphite Flakes?

I am in search of a modified Hummer's method, which can be used to synthesis graphene oxide within 6-8 hours. As I am a student it is not allowed for us to work after 5 in the laboratory. So I am...

01 August 2024 8,368 2 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

Request for Advice: Starch Metabolism Research Project?

I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...

30 July 2024 4,267 2 View

Can the limit of quantification (LOQ) of an analytical method fall outside its linear dynamic range, or must it always be within it?

Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...

29 July 2024 7,198 9 View

What is the solubility of Iron (+3) in Bismuth Oxide?

Dear all: I am modifying Bismuth Tungstate with Hematite (Fe2O3), so I would like to know about how much Iron(+3) can form a solid solution in a Bismuth Oxide matrix. Best wishes Marco

29 July 2024 2,163 1 View