Can anyone explain why there are some shere silica?

More Gibong Yoon's questions See All

Why HRLDAS need to combine snow and rain as Precipitation? Why this need hourly interpolation of solar radiation?

Dear coworkers, my questions are about HRLDAS. I am runing HRLDAS with GLDAS-based forcing data. For running offline LSM such HRLDAS, we know we need atmospheric forcing and initial data. In...

28 March 2020 8,726 3 View

Can anyone recommend a good caspase-3 antibody?

I'm looking for an antibody that can detect both procaspase-3 (>30kDa) and active caspase-3(>15kDa) using western blot analysis. Did you test this experimentally? Did you get good results using a...

20 January 2020 112 3 View

How to treat poly(I:C) (polyIC, poly-IC, polyI:C) to NS-SV-AC or other cell line?

Hi, I'm experimenting to check the effect of treating polyIC (poly(I:C), polyIC, poly-IC, polyI:C) to NS-SV-AC cell lines. But I haven't been witnessing a significant cell death after treating...

23 July 2019 1,690 1 View

Is impacting the only option for experimental modal analysis of structure with free-free boundary condition?

For the modal testing of free boundary condition structure, such as a steel beam put on the bungee cords, is it possible to apply force using the actuator, like modal shaker? I guess it is...

24 June 2019 3,806 3 View

How can I estimate the purity of a recombinant protein after purification process using size-exclusion chromatography (no standard product exists)?

I produced a recombinant chimeric protein from CHO cells and purified it using size-exclusion chromatography(SEC). Thankfully, I could get a clear peak for my target protein and separate it from...

23 April 2019 4,383 12 View

How to decide sample size for validation?

I have data having over 20000 rows. 1) I am going to use a tool to test how many error rates this tool will calculate in this data. 2) And then, I will randomly choose some data and check how...

24 March 2019 7,656 4 View

Do i have to include all the hydrogen atoms for atomic charge calculations using GROMOS force field?

Dear all, I know that GROMOS96 force filed is united atom. When calculating the atomic charge using the GROMOS96 force field, are the value calculated considering all hydrogen atom in the ligand?

16 January 2019 7,978 1 View

How can i use EMBL pride inspector?

Hellow everyone. I am supposed to use EMBL pride proteomics documents. But I don't know how to use EMBL pride inspector program. even there is no file to introduce inspector program. Up to now, I...

29 May 2018 4,754 2 View

Are there any tries or results about carbon nanotubes film prepared through vacuum thermal evaporation?

I am searching some tries related to it. But most of the search results are about a synthesis of the carbon materials itself via chemical vapor deposition (CVD).The term 'CVD' contains 'vapor' so...

19 June 2017 3,463 2 View

Looking for a polymer with specific properties

Could anyone suggest a polymer having following properties? 1. It should be easily soluble in water at 20 degree C 2. It should NOT be soluble in ethanol 3. It's melting point must be well above...

11 June 2017 3,547 5 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

What pH condition required to coat APTES on silica coated Fe3O4?

It's a hydrolysis reaction, which may produce by either acid or base-catalyzed reaction. so can anybody help me with this, which condition is suitable for this reaction?

26 February 2021 1,760 8 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View