I am reading the following paper. I had a hard time to understand CPMG and CEST and the difference between them. Is there anyone who can explain to me please
Article Structure and Dynamics of an Intrinsically Disordered Protei...
I was reading about CPMG and CEST NMR but no reviewer has written any good review for basics. I had a hard time understanding this fig 2 from above paper, and paper is quite complicated to follow
In Fig 2 a) What is R2 eff ( S ) means. I understood like this so with more and more protein y-axis value is higher so highest value 15 ( I believe axis is sec) it drops from 15 sec to 10 sec at 200 HZ then flatten out, and what does that tell you?
In Fig 2 b)
There is 2 dip, one is an excited state and another is the ground state. For the same amide excited state and ground-state chemical shifts are different. So they are exchanging in two different states is that what the author is saying?
It is about different exchange time-scale (also they are different methods). CPMG based relaxation dispersion spectroscopy relies on transversal magnetization,. CEST is based on longitudinal magnetization which makes it amenable for the study of complexes even in slow exchange. So both of them can tell you about a dark/hidden conformational state of a protein. Imagine protein that has second conformation but only of 3% population, you can not see it since the HSQC peaks are the average in the normal experiment. Using these methods you can see if some of the residues reflect the low-populated conformation. For example (b) in the middle shows 2 spikes. They are 15N shifts if major and minor conformation, normally not visible in the HSQC.
The way to find out is to Google the terms and read multiple different definitions to get the best overall understanding
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