I need your help to get some good protocol for extracting metabolites (volatile and non volatile both) from urine and serum samples. Generally I have found through literature surveys that people are using methanol to extract metabolites from serum. Can anybody suggest some good protocols for metabolite extraction from urine and serum. I would be using LC-MS & GC-MS for my studies.
Dear Khushman,
The following may be of help!
Szultka M, Pomastowski P, Railean-Plugaru V, Buszewski B. Microextraction
sample preparation techniques in biomedical analysis. J Sep Sci. 2014
Nov;37(21):3094-105. doi: 10.1002/jssc.201400621. Epub 2014 Sep 25. PubMed PMID: 25132413.
Salting-out assisted liquid-liquid extraction for bioanalysis.
Tang YQ, Weng N. Salting-out assisted liquid-liquid extraction for
bioanalysis. Bioanalysis. 2013 Jun;5(12):1583-98. doi: 10.4155/bio.13.117.
Review. PubMed PMID: 23795935.
http://europepmc.org/abstract/MED/2585385 Metabolomic method: UPLC-q-ToF polar and non-polar metabolites in the healthy rat cerebellum using an in-vial dual extraction. (PMID:25853858 PMCID:PMC4390242)
Review: Targeted Metabolite Profiling: Sample Preparation Techniques for GC-MS-Based Steroid Analysis. Krishna Chaitanya Sadanala. Mass Spectrometry Letters / V.3, No.1, 2012 year, pp.4
Doi : http://dx.doi.org/10.5478/MSL.2012.3.1.004
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