Has anyone had any successful experience generating polyclonal antibody (in rabbit)? If so, could you please share your experience and the company that you decided to generate the antibody from.
My plan is to express my protein of interest (tagged) in E. coli. The protein will be purified using AKTA and tag will be cleaved prior to sending it off. Thus the purified protein will be quite pure and can be concentrated higher than 1 mg/ml.