The dried herbal drug was grinded and than successive extracted with Soxhlet by using hexane, dichloromethane and methanol to eleminate the low-molecular-substances. The dried residue than was extracted in hot water and centrifugated. After dialysis, freeze drying and precipitation in ethanol you get the raw polysaccharides, which you can analyse after acidic hydrolyses in the monosaccharides by using HPLC, TLC or colour reactions and detection with photometry at differenz wavelength.

Or you can use different analyser kits for the monosaccharides after enzymatic decomposition, chemical coupling and photometric measurement by different wavelength against standard saccharides.

What about the phenol-sulfuric acid method for analysis of polysaccharides? Is it useful or not for analysis? Only thing is that it uses a very basic technique i.e. UV for final analysis. Please share your valuable views.