Not sure if this is a perfect procedure as I have not performed this but you might find it useful... I got this from Nugroho et al. (2014)
Peroxynitrite-scavenging activity
An assay method described by Kooy et al. [19] was used to measure the peroxynitrite-scavenging activity of the compounds, extracts, and fractions from P. aviculare. The principle of this method is to monitor the intensity of highly fluorescent rhodamine formed from non-fluorescent DHR 123 under the presence of ONOO−. The concentration of DHR 123 was 5 μM. The samples were dissolved in 10% DMSO (concentration: 5 μg/mL). The final fluorescent intensity was measured with or without the treatment of 10 μM ONOO− in 0.3 N NaOH. The fluorescence intensity of the oxidized DHR 123 was measured at the excitation and emission of 480 nm and 530 nm using a microplate fluorescence reader FL 500 (Bio-Tek Instruments Inc., Winooski, VT, USA).
The method by Kooy et al. (1994) have been used by several researchers already. So I think it is an effective method.
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