How to show a protein is being dimerized in SDS-PAGE gel?

A protein dimerizes through CTD. I have expressed CTD of protein with two different tags. Now, I want to show that these CTD of protein dimerize in a pull-down assay. But my doubt is that if the...

29 January 2021 7,529 4 View

What are the impacts of pKa1and pKa2 in drug absorption ?

Pregabalin is a crystalline solid with a pKa1 of 4.2 and a pKa2 of 10.6. what insight can i get from this aforementioned information during drug design. What is the difference between pKa1and pKa2.

28 December 2020 7,237 3 View

How to deal with negative factor loadings while performing PCA-MLR to calculate quantitative source contribution in air pollution studies ?

If I am getting some negative values in factor loadings while performing principal component analysis (PCA), then I should use that negative values to calculate daily source contribution using...

22 December 2020 5,248 1 View

What are the source markers of Naphthalene, Acenaphthylene and Acenaphthene in ambient atmosphere ?

Is any article available which reports the sources of these PAHs ?

22 December 2020 8,738 1 View

Why high infiltration in OM treated plot?

We are carrying out trial in the glasshouse where we are comparing the infiltration rate in sodic soil in two different treatments Polyacrylamide vs organic matter ( lucerne pellet). within 27...

25 November 2020 8,787 3 View

Where can one download data (csv, excel) for average daily values of AQI (air quality index) in popular cities, like, Delhi and Mumbai in India?

Air pollution has become a very big problem in Indian cities, especially Delhi. It requires immediate attention and we need to soon understand the trends, provide forecasts for future, issue...

19 November 2020 1,433 3 View

What is the best feasible method to plot DISTANCE vs LST graph?

The above question is intended to be answered in reference to the reduction of the urban heat island by the river.

22 October 2020 7,732 4 View

Which type of measuring instrument can be used to measure hot thermic fluid flow rate?

The flow rate of thermic fluid is less than 1 L/min and temperature ranges from 25 - 200 degree Celsius with a pressure limit of 5 bar.

06 October 2020 5,612 11 View

Can anyone endorse me for arXiv.org?

Hello, I need an endorsement to submit my preprints in arXiv.org. I am in the bioinformatics research field. The arXiv requires an endorsement for me because my institution doesn't provide any...

02 October 2020 1,041 4 View

How to calculate a flow rate over the broad-crest wear for this conditions?

How to find a relationship between velocity and weir landing length (X') as shown in figure?

28 September 2020 7,159 3 View

How do I measure Arabidopsis germination rate?

Hi there, I'm looking for a way to measure the Arabidopsis germination rate, like what media I should use to plant the seeds and what indicator I should see for germination. I'm planning to...

02 February 2021 4,785 3 View

What are the different subclasses in intermediate seeds?

I am currently working on an endemic tree species, that produces mature seeds (moisture content 51%) which shows both vivipary (30% of total harvested seeds from a tree) and dormancy (~40%) of...

20 January 2021 2,670 3 View

What type of false light is best for checking seed germination (in dark treatment)?

Hi I have been asked to check a weed species germination characteristics in "light" and "dark" treatments. Can someone please advise what type of "false light" I should use to check germination...

14 January 2021 5,943 4 View

What are ways to induce dormancy in cancer cell lines in vitro?

Hello, I am looking for ways to induce dormancy in several cancer cell lines in vitro (MC38, 4T1, B16F10, LLC etc.). Has anybody experience with this topic? What are common ways?

11 January 2021 5,792 2 View

What will be the appropriate data collection method and data analysis that I can employ with may experimental research on squash germination?

I am conducting an experimental research on the germination enhancement of squash affected by mechanical, chemical and thermal scarification methods..i have researched about time- to-event method...

27 December 2020 7,133 3 View

Why do the allelopathic produce a positive effect?

Following an experiment where Zea mays and Cucurbita pepo seeds were planted individually and together, results showed a positive allelopathic effect on germination and seeling lenght as both...

03 December 2020 1,875 6 View

Can anyone help with setting up the R germinationmetrics Package using multiple species and treatments?

I have 14 species, 6 temperature settings and two treatments to examine germination responsiveness over 59 days. I want to start with some basic info for plotting (t50, Gmax etc). I want to...

18 November 2020 7,160 4 View

In arid regions, what environmental factors have a greater effect on the establishment and germination of rangeland plants?

In arid regions, what environmental factors have a greater effect on the establishment and germination of rangeland plants? Describe the effectiveness of the priority

16 November 2020 3,642 9 View

What is the index of germination and howa calculate it??

16 November 2020 4,856 8 View

How to equalise scale (length ) in different size images?

I have 3 images of plant germination and each image is of different dimensions. I resized all of them using paint to 600 (width) X 450 (length) pixels. But the scale size in the 3 images is of...

04 November 2020 4,134 3 View