I'm submitting a partial nucleotide sequence. I'm facing a problem at the last step where to add Coding Sequence(CDS)feature I have to mention the reading frame.Since I'm reporting a mutated sequence my original reading frame is changed.Moreover I'm also not sure about the addition of nucleotide interval feature.
Trim your sequence to start from the ATG.. or specify the position when the drop box appears in sequin
I have already solved the problem using SEQUIN,however the CDS feature of BankIt is not applicable to my sequence as it contains a frameshift mutation causing a change in CDS. I therefore included an explanatory mail with my sequence file and then it got accepted.
hello
this is my problem too!?
How do I add a feature annotation when submitting a sequence in GenBank?
You can use the BankIt tool in which under "other features" option the "Variation submission" you can select from drop down list and annotate your sequence. Please refer the GenBank Submission Handbook for more details ( https://www.ncbi.nlm.nih.gov/books/NBK51157/pdf/Bookshelf_NBK51157.pdf )
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