18 November 2014 1 2K Report

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Minimum concentration of protein to be injected for detectable peaks of protein in 16/60 Sephacryl S-100 HR column for SEC-FPLC?

Hello Peers, Warm wishes to all. I am a new user of the Cytiva AKTA Start system for SEC with a column, HiPrep 16/60 Sephacryl S-100 HR (120 ml column volume). I am not sure about the minimum...

03 June 2024 7,713 2 View

How long and at what temperature can I store the roots for analyzing morphological traits?

I want to analyze the morphological traits of Ficus hispida roots using Whinrhizo app. How long and at what temperature can I store them before scanning?

11 April 2024 9,150 1 View

How to split molecules into test and training set in 3D-QSAR?

Hi peers and experts! I am new to 3D-QSAR and tried to build a model based on 34 molecules, split into 10 test and 24 training sets. In the end, a poor model is evident from r2 and q2 values....

08 April 2024 2,785 6 View

What statistical approach is used for paired sample study design in MetaboAnalyst?

For unpaired sample test, t-test or ANOVA is used to compare differences between groups. What statistical approach is used for paired samples in MetaboAnalyst?

15 September 2023 8,567 1 View

Can anyone tell me how to run TVP VAR model and Spillover Asymmetry Measure (SAM) in R?

Hi I am trying to run TVP VAR model by Antonakakis, N., Chatziantoniou, I., & Gabauer, D. (2020). Refined measures of dynamic connectedness based on time-varying parameter vector...

16 April 2023 5,624 5 View

How much blood sample must be collected for PBMC isolation?

I need to isolate total RNA from the PBMC after that.

14 December 2021 2,170 6 View

JMP Pro 14 and one way ANOVA?

I have to analyse the data for one way ANOVA using JMP pro 14 software. Anyone dealt with this software or has any kind of experience. Kindly share it. Thanking you in advance.

23 September 2020 870 6 View

How to interpret the results obtained from X-ray diffraction (XRD)?

I prepared the gold nanoparticles using plant extract and characterized using XRD. Result displays four prominent diffraction peaks indexed as (111), (200), (220) and (311) miller indices which...

28 June 2019 8,075 10 View

Anyone please name a few good online softwares for drawing density or heat map for a particular country statewise ?

I would like to know the name of a few good and reliable geographical map drawing online freely available software which can be used for generating density based map or heat map of a particular...

23 June 2019 3,045 3 View

What is the minimum nuclear lysate fraction concentration is required to set up a Co-IP?

My protein size is 240kDa and I am interested in identifying the interacting target protein at endogenous level. I have earlier tried using the whole cell lysate but 4-5 mg of MEF cell lysate do...

21 May 2019 7,226 0 View

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