It is easy to perform procedures for sex inversion (sex reversal) using enclosed facilities. The procedures for the sex reversal of open water fish depends on a lot of factors. What species of fish will you be using and at what stage of the fish will you administer sex reversal? For species like tilapia, sex reversal is performed by feeding them with hormone-treated feeds for 17-21 days (at fry stage) . There are also research conducted by immersing eggs in hormone solution for 24-96 hours. You may try immersing the fry / egg first in a hormone solution.