Why do cells die after adding MTT dye?

I was doing MTT assay, and in the last day before adding the MTT; the cells looked good (or so I think), but after aspiration of the drug and addition of MTT dye I noticed that most cells seemed...

07 February 2021 3,846 4 View

How can I stain 3d printed cells in hydrogel without damaging the gel?

I tried staining my cells in a gel using standard protocols with 4PF% for fixation and triton-X or saponin for permeabilization but the gel always gets damaged throughout the steps, and at the end...

17 January 2021 4,170 1 View

Why do I get low absorbance in MTT assay?

I have been trying to do MTT assay using HCT-116 cells but I have been getting very low absorbance readings (absorbance is usually 0.25 and below) even though iam seeding 30,000 cell/ well. I have...

16 November 2020 3,588 3 View

Can somedbody advice on how to deal with various lingering covid-19 symptoms especialpersistent tiredness?

I contracted the infection in mid May.My symptoms included runny nose,skin,widspread skin tinglings,slight headach,profuse sweating,and extreme tiredness that I was not able to walk for few meters...

12 September 2020 2,385 4 View

Is there a simple method to use pslegend (GMT) to create a legend for 2-D plot?

I am new for Generic Mapping Tool (GMT), but I would like to work through a few simple examples of generating legends (ie. multiple points or lines plotted on a single figure using psxy). In the...

26 April 2018 8,197 3 View

How to use high precise geoid for mineral exploration?

Recently, many scientific applications such as: In geology, the inversion of the geoid is used for Petroleum Exploration. I am looking for studies and research in this field

01 January 2018 2,539 4 View

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Read 12 answers by scientists to the question asked by Saifeldin Mohamed Khair Abdelrahim on Sep 20, 2016

19 September 2016 5,921 10 View

What is the idea of your best research work in human nutrition?

Read 5 answers by scientists to the question asked by Saifeldin Mohamed Khair Abdelrahim on May 12, 2016

11 May 2016 4,903 4 View

What are the different sites for downloading best resolution (below 20 m) for free remote sensing imagery?

I am looking for satellite image used for academic purposes

10 November 2015 6,722 8 View

How can I calculate sample size in clinical control trial (qualitative study)?

28 August 2015 1,889 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Why cannot world and community leaders be gentle? Where did ethics of care go?

The whole world is in a mess and paused. We all are tired of seeing hypermasculinized and destructive leaderships all around us. This world has seen enough of the aggressive patriarchal social...

23 February 2021 292 7 View

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22 February 2021 9,932 11 View

How to perform Haldane correction on SPSS?

I was wondering if anyone knew how to perform the Haldane correction for SPSS (this is the definition of the correction -...

21 February 2021 2,882 1 View

Is it okay to mention you distributed your qualitrics study link through social media sites?

Just want to be clear and know it is okay to say I have distributed the qualitrics link through social media such as Facebook, Instagram and Twitter to ensure my target audience is reached in my...

21 February 2021 5,143 2 View

How COVID-19 has affected mental health?

The pandemic has led to depression and anxiety and its negative effects shouldn't be ignored. However, it has also led some people to become more resilient! What do you think? Thank you.

21 February 2021 4,733 19 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View