Can any one help me on how effectively detect hif1 alpha in mouse PASMcs or mouse cell line exposed to hypoxia?

29 April 2020 0 5K Report

I tried several times with different ab ( Cayman, BD) and didn’t get good results, i always don’t have induction of hif1 a in hypoxia and have prominent expression in normoxia also.

i want to know technical tricks that should I follow to obtain good results.

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