Bisolvent extraction method and colorimetric method are used for the determination of solanine and solanidine from potatoes. A high-performance thin-layer chromatographic (HPTLC) method is also used to determine the glycoalkaloids alpha-solanine and alpha-chaconine in potatoes. Alpha-solanine and alpha-chaconine are extracted from dehydrated potatoes with boiling methanol-acetic acid (95 + 5, v/v). The analytes are separated on a Silica Gel 60 F254 HPTLC plate by a saturated mixture of dichloromethane-methanol-water-concentrated ammonium hydroxide (70 + 30 + 4 + 0.4, v/v), which is used for vertical development of the plate up to a distance of 85 mm. For visualization, the plate is dipped 3 times into a modified Carr-Price reagent, 20% (w/v) antimony(III) chloride in acetic acid-dichloromethane (1 + 3, v/v), and subsequently heated on a hot plate at 105 degrees C for 5 min.

Dear Rachid

Please find the methods in attachment.

Remaining ones

Oh thank you very much for your answers

Dear Neeraj thanks

Dear Ravi kant thank you

I am also trying to quantify solanine and chaconine from freeze dried potatoes. I am about to start working on a Quechers method which I found in literature. Attached is the paper.

Thank you very much dear Ujwal

Just a quick update, the method worked perfectly with recoveries between 85-105%. I did not have to use internal standard.