IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

How to delete a copy of a paper, and how to delete a co-author's name?

I mistakenly uploaded a copy of the same paper and entered my alternative name. How to delete them out? (I couldn't navigate to the right buttons to do it. So from where to where is what I don't...

07 January 2021 7,694 6 View

How can Meta-Analysis be applied to the Life cycle assessment (LCA) of Wastewater treatment Plants (WWTPs)?

I am new into the meta-analysis concept. Having done a little reading and watched videos on YouTube, I understand there are different kinds of meta-analysis. And the type of summary statistics or...

29 December 2020 3,677 3 View

Best Way to Preserve Nitrifying Bacteria in Liquid Culture?

I have a heterogeneous culture of nitrifying bacteria that was originally isolated from the field. It has been kept alive for several months in a bioreactor. I want to pull some of this...

18 December 2020 5,411 4 View

HPLC data show negative peak on the blanks, please can I solve the problem?

09 November 2020 5,884 5 View

Any molecular beacon producing company to recommend ?

Hello, For one of my projects I would like to test a Live RNA imaging using molecular beacons such as in this paper : Molecular Beacon-based Detection and Isolation of Working-ty... As we...

28 October 2020 9,457 2 View

MsaClustalW() Take ages. What to do ?

Hello, I'm new to the bioinformatics world and would like a feedback on which MSA tool to use. I want to find a consensus sequence in about 18000 sequences (from marine biology samples) from...

19 October 2020 2,607 2 View

What is the best way to store PBMC after isolation?

So I need to store these for 4 weeks at least, before I do a Dyna bead negative T cell isolation on it. What should I store it in FBS? or DMSO and at 4 degrees? or is -20 not good for the cells?

26 July 2020 2,339 3 View

Best way to preserve non-sporulating bacteria?

What is the best way to preserve non-sporulating bacteria for further study?

20 May 2020 5,532 5 View

Best way to preserve heterogeneous culture of non-sporulating bacteria?

I have a culture of heterogeneous bacteria that will NOT form endospores. This bacteria was originally isolated in nature and is a complex culture of multiple species. I want to be able to...

20 May 2020 8,369 1 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Is there an open source free software for text mining and sentiment analysis?

I am looking for a free software for text mining and sentiment analysis for my research on customer review mining (it involves calculating polarity of attributes,opinion oriented information...

25 February 2021 1,920 6 View

What software programs can directly simulate Additive Manufacturing Directed Energy Deposition (DED)?

I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....

24 February 2021 4,428 3 View